| 王利利,涂彧,周菊英,崔凤梅,徐晓婷.放疗对肿瘤患者外周血淋巴细胞hprt基因位点的影响[J].中华放射医学与防护杂志,2004,24(3):205-206 |
| 放疗对肿瘤患者外周血淋巴细胞hprt基因位点的影响 |
| Effects of radiotherapy on hprt gene in malignant tumor patients |
| 投稿时间：2003-10-16 |
| DOI： |
| 中文关键词: 放射治疗 hprt基因 突变频率 多核细胞法 |
| 英文关键词:Radiotherapy hprt gene Mutation frequency Poly-nuclear cell assay |
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| 中文摘要: |
| 目的 了解放疗对肿瘤患者外周血淋巴细胞hprt基因位点的影响, 探讨hprt基因突变分析作为放疗致机体辐射损伤监测的可行性。方法 采用多核细胞法研究放疗对人外周血淋巴细胞hprt基因位点的影响及其突变频率的变化。结果 放疗可诱发人外周血淋巴细胞hprt基因位点突变, 突变频率随放疗累积剂量的增加而升高。在同一剂量点, 鼻咽癌病人与乳腺癌病人的hprt基因突变频率统计学上没有显着差异。肿瘤病人的hprt基因突变频率均较正常对照组高。结论 hprt基因位点突变频率分析可作为放疗致机体辐射损伤监测的生物学标志。 |
| 英文摘要: |
| Objective To study the effect of radiotherapy on hprt gene in peripheral blood lymphocytes,and explore the possibility of radiotherapy-induced genetic damage detected by the frequency of hprt locus mutation. Methods A simple,rapid and sensitive poly-nuclear cell assay was used to investigate the frequency of hprt locus mutation in malignant tumor patients prior to and after receiving radiotherapy with a cumulative dose of 54-72 Gy. Results The results showed that radiotherapy could induce hprt locus mutation in malignant tumor patients.It was found that there was a close correlation between the frequency of hprt locus mutation and the cumulative dose:that is the larger the cumulative dose,the severe the genetic damage.At the same dose,the mutation frequency of nasopharyngeal carcinoma patients was close to that of the breast cancer patients.The frequency of hprt in malignant tumor patients was higher than that of normal,healthy people. Conclusion The frequency of hprt locus mutation may be expected to be used as a potential biological indicator for genetic damage induced by radiotherapy. |
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