| 刘芬菊,陈剑,李冰燕.激光共聚焦显微镜观察(60)Co γ射线对CNI-H446 DNA/RNA比值及三维结构的影响[J].中华放射医学与防护杂志,2004,24(3):203-204 |
| 激光共聚焦显微镜观察(60)Co γ射线对CNI-H446 DNA/RNA比值及三维结构的影响 |
| Effects of irradiation with 60Co γ-rays on the ratio of DNA and RNA in CNI-H446 cells and its three-dimensional structure by laser scanning confocal microscopy |
| 投稿时间：2003-10-16 |
| DOI： |
| 中文关键词: 共聚焦显微镜 CNI-H446细胞株 DNA RNA 60Co γ射线 |
| 英文关键词:Laser scanning confocal microscope (LSCM) CNI-H446 strain DNA RNA 60Co γ radiation |
| 基金项目:苏州大学医学发展基金重大项目资助(2003) |
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| 中文摘要: |
| 目的 探讨电离辐射对小细胞肺癌细胞株(CNI-H446)DNARNA代谢比值的影响及观察细胞三维结构的改变。方法 利用共聚焦显微镜双重荧光标记、全自动三维图像分析CNIH446细胞受到不同剂量60Coγ射线照射后DNA和RNA含量的比值。结果 对照组细胞DNARNA比值为1.15±0.07,当受到1Gy以上剂量照射时, DNA含量明显降低, 诱导RNA合成逐渐增加, DNA降低的程度大于RNA,1,3Gy组与对照组相比差异具有非常显着性(P<0.01).三维图像显示RNA聚集, RNA荧光强度明显增强, 而DNA均匀散在于细胞内。当剂量>8Gy时, RNA合成受到显着抑制, 两者比值明显增加, 细胞体积增大, 其比值与照射剂量存在一定的联系。结论 CNIH446细胞受到不同剂量60Coγ射线照射后DNARNA之比及细胞大小随着照射剂量的增加不断发生变化。 |
| 英文摘要: |
| Objective To investigate the effect of γ-radiation on DNA and RNA metabolism in cells of small cell lung cancer (SCLC) strain (CNI-H446) and their three-dimensional structure. Methods Laser scanning confocal microscopy (LSCM) and acridine orange (AO) staining was used to investigate the DNA/RNA changes in the cells,and automatic three-dimensional image scan was used to fine the change of its structure. Results The DNA/RNA of the control group was 1.15±0.07.When the dose of γ-radiation was more than 1 Gy,DNA decreased obviously and the extent of the decrease of DNA was larger than that of RNA.The values of DNA/RNA of the groups that were irradiated with 1 Gy and 3 Gy were significantly different from those of the control group (P<0.01).The three-dimensional image manifested that after large dose of irradiation,the RNA in the cells gathered obviously while DNA were disrupted.When the dose was 8 Gy,the value of DNA/RNA was not different from that of the control group.When the dose was 8 Gy or higher,RNA was suppressed as seriously as DNA,and the value of DNA/RNA increased.There is certain relationship between the value of DNA/RNA and the dose of radiation. Conclusion After irradiated with different dose of γ-radiation,the ratio of DNA and RNA and the size of cells would change correspondingly. |
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