| 曹建平,E. Fritz,郑斯英,朱巍,罗加林,周献锋,王明明.酵母TEL1基因对辐射诱发A-T细胞染色体畸变的影响[J].中华放射医学与防护杂志,2004,24(3):192-195 |
| 酵母TEL1基因对辐射诱发A-T细胞染色体畸变的影响 |
| Effect of yeast TEL1 gene expression in A-T cells on chromosome aberration induced by radiation |
| 投稿时间：2003-10-16 |
| DOI： |
| 中文关键词: A-T细胞 酵母TEL1基因 电离辐射 染色体畸变 |
| 英文关键词:Ataxia-telangiectasia Yeast TEL1 gene Ionizing radiation Chromosome aberration |
| 基金项目:国家自然科学基金资助项目(30170288); 教育部留学回国人员科研启动基金资助项目(教外司2002247) |
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| 中文摘要: |
| 目的 研究酵母TEL1基因在毛细血管扩张运动共济失调症(ataxia-telangiectasia,A-T)A-T细胞中的异位表达对辐射诱发A-T细胞染色体畸变的影响。方法 稳定转染酵母TEL1全基因序列、酵母TEL1基因片断和pRep5空载体的A-T-TEL1、A-T-deltaTEL1、A-T-rep、GM-rep细胞分别在含100μgml潮霉素的DMEM培养液中培养;A-T细胞和正常对照的GM639细胞分别在DMEM培养液中常规培养。应用60Co源对处于指数生长期的上述细胞进行γ射线照射, 吸收剂量分别为0,1和3Gy.应用常规染色体技术制片, 并进行染色体畸变分析。结果 传代培养的A-T-TEL1、A-T-delta-TEL1、A-T-rep、A-T、GM639、GM-rep细胞的染色体数目均是非整倍体;无论是A-T细胞染色体自发畸变率还是辐射诱发的染色体畸变率均明显高于正常对照的GM639纤维母细胞;A-T细胞的高染色体畸变率主要是由于染色体双着丝粒体和染色体断片所至的染色体型畸变;稳定转染酵母TEL1全基因序列的A-TTEL1细胞染色体自发畸变率与A-T细胞之间差异无显着性(P>0.05);辐射诱发A-TTEL1细胞的染色体畸变率明显低于A-T细胞(P<0.01);辐射诱发A-Tdelta-TEL1、A-T-rep细胞染色体畸变率则高于A-T细胞。结论 酵母TEL1基因在A-T细胞中的异位表达, 能够明显降低电离辐射诱发。 |
| 英文摘要: |
| Objective To analyze the effect of yeast TEL1 gene expression in human A-T (ataxia-telangiectasia) cells on chromosome aberration induced by radiation. Methods Yeast TEL1 gene (TEL1rep),TEL1 fragment (deltaTELrep),pRep5 vector were stably transfected into A-T cells and pRep5 vector was stably transfected into GM639 cells.respectively.The stably transfected A-T and GM639 cells,namely,AT-TEL1,AT-deltaTEL1,AT-0rep,GM-rep,were grown selectively in Dulbecco's minimal essential medium (DMEM) containing 100 μgml hygromycin,while non-transfected A-T and GM639 cells were grown in DMEM containing 15%-20% FBS. 60Co γ-rays were used to irradiate the above cells at exponential phase.The irradiation dose was 0,1,3 Gy,respectively.A hundred metaphases were analyzed for microscopically detectable chromosome type and chromatid type aberrations. Results The chromosome numbers of all transfected,and non-transfected A-T,GM639 fobroblasts were aneuploid.Both spontaneous and radiation-induced chromosome aberration frequencies of A-T cells were higher than those of GM639 control cells.There is no significant difference in the spontaneous chromosome aberration between TEL1 gene stably transfected A-T cells (AT-TEL1) and non-transfected A-T cells (P>0.05).Chromosome aberration frequencies induced by radiation in A-TTEL1 cells were significantly lower than those of A-T cells (P<0.01).However,chromosome aberration frequencies induced by radiation in AT-deltaTEL1 and AT-rep cells were higher than those of A-T cells. Conclusion The expression of yeast TEL1 gene in human A-T cells can significantly decrease the chromosome aberration frequencies induced by radiation. |
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