中华放射医学与防护杂志

朱飞鹏,王洪复,高林峰.α骨化醇对肾辐射损伤大鼠骨代谢影响的保护作用[J].中华放射医学与防护杂志,2004,24(2):125-127.ZHU Fei-peng,WANG Hong-fu,GAO Lin-feng.Effect of alpha-calciferol on bone metabolism in rats with radiation injury to kidney[J].Chin J Radiol Med Prot,2004,24(2):125-127

α骨化醇对肾辐射损伤大鼠骨代谢影响的保护作用

Effect of alpha-calciferol on bone metabolism in rats with radiation injury to kidney

投稿时间：2003-05-16

DOI：

中文关键词: 辐射肾脏骨代谢 α骨化醇

英文关键词:Radiation Kidney Bone metabolism Alpha-calciferol

基金项目:

作者	单位
朱飞鹏	200032 上海, 复旦大学放射医学研究所骨代谢研究室
王洪复	200032 上海, 复旦大学放射医学研究所骨代谢研究室
高林峰	200032 上海, 复旦大学放射医学研究所骨代谢研究室

摘要点击次数: 3249

全文下载次数: 1973

中文摘要:

目的观察α骨化醇对肾辐射损伤骨质疏松大鼠骨质量的保护作用。方法 6月龄健康雄性SD大鼠30只,随机分为模型(Ⅱ),假手术(Ⅰ)和α骨化醇治疗(Ⅲ)3组,每组10只。以15Gy¹³⁷Csγ射线照射大鼠肾脏局部制备肾辐射损伤模型,照射后1周,α骨化醇治疗组给予萌格旺0.1μg·kg体重^-1·d^-1,以1%羧甲基纤维素钠(CMC)制成混悬液灌胃,1ml/只,模型组以1%CMC灌胃,连续3个月。实验结束后,各组大鼠取L₁～L₄腰椎及右侧股骨,分别测定骨密度(BMD)、骨形态计量和生物力学等指标。结果与Ⅰ组比较,Ⅱ组的腰椎骨密度明显降低,骨小梁体积、厚度减少,骨小梁间距增宽,矿化沉积率,股骨、腰椎的最大载荷明显降低(P<0.05,P<0.01)。α骨化醇治疗组与模型组比较,腰椎骨密度明显提高(P<0.01),骨小梁体积、宽度显著增加,骨小梁间距变小(P<0.05,P<0.01),矿化沉积率,股骨、腰椎的最大载荷明显提高(P<0.05,P<0.01)。结论 α骨化醇对肾局部辐射损伤导致骨质疏松大鼠骨质量有明显改善作用。

英文摘要:

Objective To study the protective effect of alpha-calciferol on bone metabolism in rats with radiation injury to kidney. Methods Six-month-old male SD rats were randomly divided into three groups:model group, sham group, and alpha-calciferol (Bone-One) group, 10 rats each. The model group was developed by irradiation of rat kidneys with 15 Gy of Cs ¹³⁷ γ-rays. One week after radiation, alpha-calciferol group was given orally Bone-One, 0. 1 μg·kgBW ^-1·d^-1, suspended in 1% CMC, 1 ml a rat for 90 days. BMD of L _1-4, bone histomorphometry, bone biomechanical properties were measured in all rats at the end of experiment. Results BMD of L_1-4 was significantly lowered, volume and thickness of trabecular bone were significantly decreased, space of trabecular bone increased, ineral apposition rate (MAR), maximal load of femur and lumbar vertebra decreased (P<0. 05, P <0. 01) in model group, compared with sham group. In alpha-calciferol group, BMD of L_1-4, volume and thickness of trabecular bone increased, space of trabecular bone decreased significantly (P <0. 05, P <0. 01), MAR, maximal load of femur and lumbar vertebra increased significantly ( P <0. 05, P <0.01), compared with model group. Conclusion Alpha-calciferol improves disturbance of bone metabolism in rats with radiation injury to kidney.

HTML 查看全文查看/发表评论下载PDF阅读器

关闭