| 邹跃,周湘艳.淋巴细胞DNA双链断裂用于放射损伤检测的可行性研究[J].中华放射医学与防护杂志,2004,24(2):120-122.ZOU Yue,ZHOU Xiang-yan.Detection of DNA double-strand break of lymphocytes as a biological marker in radiation injury[J].Chin J Radiol Med Prot,2004,24(2):120-122 |
| 淋巴细胞DNA双链断裂用于放射损伤检测的可行性研究 |
| Detection of DNA double-strand break of lymphocytes as a biological marker in radiation injury |
| 投稿时间：2003-10-08 |
| DOI： |
| 中文关键词: 辐射 中性彗星电泳 淋巴细胞 DNA双链断裂 凋亡 |
| 英文关键词:Radiation Neutral comet assay Lymphocyte DNA double strand break Apoptosis |
| 基金项目:全军“十五”科研规划课题(01MB054) |
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| 中文摘要: |
| 目的 探讨受照后淋巴细胞DNA双链断裂和凋亡作为放射损伤生物学检测指标的可行性。方法 采用pH值中性彗星电泳方法,检测人外周血离体照射01～4Gy后6h和24h淋巴细胞彗星率和彗星尾长,并观察其量效关系。结果 照后6h和24h彗星率呈剂量依赖性上升,反应剂量阈值分别为06Gy和01Gy,r分别为0967和0927;照后6h彗星尾长亦呈剂量依赖性上升,r为0847。结论 中性彗星电泳法结果提示在照射剂量和淋巴细胞彗星率及彗星尾长间存在较紧密的相关性。 |
| 英文摘要: |
| Objective To investigate the possibility of applying the DNA double strand break and apoptosis of lymphocytes to biological assay in radiation injury. Methods Comet rates and comet tails were detected using neutral comet assay at 6 h and 24 h following in vitro 0. 1-4 Gy γ radiation of whole blood. Results The comet rates increased in a dose-dependent manner. The dose thresholds of comet rates at 6 h and 24 h were 0.6 Gy and 0.1 Gy, respectively. The correlation coefficients were 0.967 and 0.927, respectively. The comet tails at 6 h also increased dose-dependently with a correlation coefficient of 0.847. Conclusion The results of neutral comet assay indicates that there is close correlations between doses and comet rates and tails. |
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