| 潘耀振,孙诚谊,王玉芝.脂质体介导的IGF-1R反义寡核苷酸对胰腺癌细胞生长的抑制作用[J].中华放射医学与防护杂志,2004,24(2):114-116.PAN Yao-zhen,SUN Cheng-yi,WANG Yu-zhi.Growth inhibition of human pancreatic cancer cells by lipofection mediated IGF-1R antisense oligodeoxynucletides in combination with ionizing radiation[J].Chin J Radiol Med Prot,2004,24(2):114-116 |
| 脂质体介导的IGF-1R反义寡核苷酸对胰腺癌细胞生长的抑制作用 |
| Growth inhibition of human pancreatic cancer cells by lipofection mediated IGF-1R antisense oligodeoxynucletides in combination with ionizing radiation |
| 投稿时间：2003-05-17 |
| DOI： |
| 中文关键词: 电离辐射 脂质体 细胞凋亡 反义寡核苷酸 基因转染 |
| 英文关键词:Ionizing radiation Lipofectin Apoptosis Antisense oligodeoxynucletides Gene transfection |
| 基金项目:贵州省科学技术基金资助项目(黔基合计字20023106) |
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| 中文摘要: |
| 目的 研究照射后脂质体(lipofectin)介导的IGF1R反义寡核苷酸(ASON)体外抑制人胰腺癌细胞PC3的生长情况。方法 体外60Coγ射线照射后,观察PC3细胞的集落形成情况,以了解其辐射敏感性及确定合适的辐射剂量。用MTT法比较脂质体介导的ASON直接转染与联合辐射转染对PC3细胞的抑制效果,用流式细胞仪和RTPCR方法检测两种不同方法对PC3细胞凋亡及IGF1RmRNA表达的调控情况。结果 联合照射的脂质体介导的ASON组明显优于未照射组(P<0.05)。PC3细胞的凋亡率及IGF1R的mRNA水平亦均明显高于和低于未照射组(P<0.05)。结论 电离辐射可促进脂质体介导的IGF1R反义寡核苷酸转染,显著地降低IGF1R的mRNA水平,诱导PC3细胞的凋亡。 |
| 英文摘要: |
| Objective To study the growth inhibition of human pancreatic cancer cells (PC-3) by lipofectin-mediated and ionizing radiation improving transfection of IGF-1R antisense oligodeoxynucletides(ASON) in vitro. Methods Colonigenicity of PC-3 cells in vitro after 60Co γ-radiation was observed for ascertaining their radiosensitivity and optimal radiation dose was selected according to the radiation sensitivity. PC-3 cells were transfected by two ways:①by lipofection-mediated IGF-1R ASON combined with ionizing radiation. ②by lipo-ASON alone without ionizing radiation. Cell growth was assessed by MTT method. The expression of IGF-1R at mRNA level was examined by RT-PCR. Flow cytometry was used to demonstrate apoptotic changes in lipo-ASON-treated cells. Results The inhibitory efficiency of lipo-ASON combined with ionizing radiation was higher than that without ionizing radiation (P< 0.05). The apoptotic efficiency and the decreased level of IGF-1R at mRNA were significantly improved ( P< 0.05). Conclusion Lipofectin-mediated and ionizing radiation-promoted transfection of IGF-1R antisense oligodeoxynucletides(ASON) significantly decreases IGF-1R at mRNA level and induces apoptosis of human pancreatic cancer cells in vitro. |
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