中华放射医学与防护杂志

孙新臣,王俊杰,甄永苏,等.粉防己碱对放射线的增敏作用与机理研究[J].中华放射医学与防护杂志,2004,24(2):99-101.SUN Xin-chen,WANG Jun-jie,ZHEN Yong-su,et al.Enhancement of radiosensitivity by tetrandrine is associated with abrogation of cell cycle checkpoint[J].Chin J Radiol Med Prot,2004,24(2):99-101

粉防己碱对放射线的增敏作用与机理研究

Enhancement of radiosensitivity by tetrandrine is associated with abrogation of cell cycle checkpoint

投稿时间：2003-03-12

DOI：

中文关键词: 粉防己碱增敏作用 X射线

英文关键词:Tetradrine Radiosensitivity X-rays

基金项目:国家杰出青年基金资助项目(30025043);国家重大专项基金资助项目(2002AA2Z346D);国家“973”基金资助项目(2002CB513108)

作者	单位	E-mail
孙新臣	100050 中国医学科学院中国协和医科大学医药生物技术研究所	shaor@public3.bta.net.cn
王俊杰	100050 北京北京大学第三医院
甄永苏	100050 中国医学科学院中国协和医科大学医药生物技术研究所
邵荣光	100050 中国医学科学院中国协和医科大学医药生物技术研究所

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中文摘要:

目的探讨粉防己碱(Tet)在结肠癌中对X射线的增敏作用及机理。方法采用克隆形成测定法、流式细胞术、Western Blotting与分裂指数法检测Tet在体外对X射线的增敏作用与机理。使用肿瘤生长延缓实验检测Tet在体内对X射线的增敏作用。结果结肠癌HT-29细胞在受到X射线照射后,明显阻滞于G₂M期;Tet可以清除这种阻滞,并起到显著的增敏作用,其增敏比为1.63。进一步研究显示,HT-29细胞在受到X射线照射后,其Chkl蛋白表达水平升高,Cyclin B1表达水平明显降低,分裂指数也明显降低;在用Tet处理后,Chkl蛋白表达水平降低,Cyclin B1的表达水平明显增高,分裂指数也增高。体内实验结果表明,Tet明显引起受照射小鼠结肠癌C26生长延缓。Tet与X射线联合使用组的肿瘤生长延缓天数是单用X射线组的1.63倍,对照组的2.82倍。结论 Tet是一种细胞周期关卡清除剂,体外和体内对X射线都有明显的增敏作用。

英文摘要:

Objective To investigate the radiosensitizing effect of tetrandrine (Tet) on human colon carcinoma HT-29 cells and its mechanism. Methods Clonogenic assay, flow cytometry and Western blotting were preformed in the experiments. In an animal model, tumor growth delay assay was used to determine the radiosensitivity. Results X-rays radiation markedly induced G₂/M phase arrest in HT-29 cells. Tet abrogated the G₂/M arrest induced by X-rays, and enhanced the cytotoxicity of X-irradiation by 1. 63-fold. Furthermore, X-rays increased expression of Chkl protein and decreased Cyclin B protein levels. Tet prevented increase of Chkl protein kinase and blocked decrease of Cyclin B1 protein. Mitotic index measurement showed that Tet dramatically stimulated X-irradiated cells to enter mitosis. The data showed that Tet enhanced suppression of the growth of colon carcinoma C26 by X-rays in BALB/c mice. Conclusion Tet is a potent abrogator of cell cycle checkpoint and enhances radiosensitivity in vitro and in vivo.

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