| 吴晓旻,杨明亮,黄晓兰,阎俊,罗琼.褐藻糖胶对照射大鼠淋巴细胞凋亡及相关基因表达影响[J].中华放射医学与防护杂志,2004,24(1):33-34 |
| 褐藻糖胶对照射大鼠淋巴细胞凋亡及相关基因表达影响 |
| Effect of fucoidan on lymphocyte apoptosis and apoptosis-related gene expression induced by irradiation |
| 投稿时间：2003-07-30 |
| DOI： |
| 中文关键词: 褐藻糖胶 照射 淋巴细胞 Bcl-2 Bax |
| 英文关键词:Fucoidan Irradiation Lymphocyte Bcl-2 Bax |
| 基金项目:湖北省科技厅重点攻关资助项目(2001AA307B) |
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| 中文摘要: |
| 目的 研究褐藻糖胶(fucoidan)对照射诱导的大鼠脾淋巴细胞凋亡及相关蛋白Bcl2和Bax表达的影响。方法 通过灌胃方式给予Wistar大鼠不同剂量的褐藻糖胶,连续给药10d后行一次性9.0Gy60Coγ射线照射;采用TUNEL、流式细胞术等观察大鼠淋巴细胞凋亡的变化,用免疫组织化学法检测其Bcl2和Bax蛋白的表达。结果 照射结束18h后大鼠脾淋巴细胞凋亡明显增加,脾淋巴细胞Bcl2/Bax蛋白比值显著下降。一定剂量的褐藻糖胶对照射诱导的大鼠脾淋巴细胞凋亡和Bcl2/Bax比值下降具有显著的拮抗作用,且呈明显的剂量效应关系。结论 褐藻糖胶对照射诱导的大鼠脾淋巴细胞凋亡具有显著的抑制作用,其机理与调节脾淋巴细胞Bcl2和Bax蛋白表达有关。 |
| 英文摘要: |
| Objective This study was conducted to explore the effects of fucoidan on rat splenic lymphocyte apoptosis and its Bcl-2 and Bax expression induced by 60Coγ-rays. Methods Rats were provided with different doses of fucoidan before whole-body irradiation with 9.0 Gy 60Coγ-rays.TUNEL and flow cytometry were used to investigate the effects of fucoidan on splenic lymphocyte apoptosis and immunohistochemical technique was used to detect the Bcl-2 and the Bax expression. Results Irradiation caused increase of apoptosis in rat splenic lymphocytes obviously,and the ratio of Bcl-2/Bax protein decreased significantly.Fucoidan could markedly inhibit the effects of radiation on apoptosis and the ratio of Bcl-2/Bax protein in a dose-dependent manner. Conclusion Fucoidan could inhibit rat splenic lymphocyte apoptosis induced by radiation,and its mechanism could be associated with its regulation on expressions of Bcl-2 and Bax proteins in splenic lymphocyte. |
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