| 叶江枫,戚好文,梁军,蒙育林,赵锋.ET-1在放射性肺损伤中的改变及维甲酸、氟伐他汀的治疗作用[J].中华放射医学与防护杂志,2004,24(1):30-32 |
| ET-1在放射性肺损伤中的改变及维甲酸、氟伐他汀的治疗作用 |
| Changes of endothelin-1 and curative effect of retinoic acid and fluvastatin in lung injury induced by thoracic radiation |
| 投稿时间：2003-03-11 |
| DOI： |
| 中文关键词: 放射性肺损伤 氟伐他汀 维甲酸 内皮素-1 |
| 英文关键词:Radiation-induced lung injury Fluvastatin Retinoic Acid Endothelin-1 |
| 基金项目:第四军医大学西京医院高新技术基金资助项目(200120) |
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| 中文摘要: |
| 目的 探讨血浆及肺组织中ET-1在放射性肺损伤中的改变及维甲酸、氟伐他汀对其治疗作用。方法 健康雌性SD大鼠100只随机分为5组:正常对照组(N)、单纯照射组(S)、维甲酸治疗组(W)、维甲酸+氟伐他汀治疗组(L)、地塞米松治疗组(D),每组20只。后4组行直线加速器全胸部照射,照射后次日维甲酸治疗组灌服维甲酸,L组灌服维甲酸、氟伐他汀,D组灌服地塞米松,于照射后5,15,30,60d采血测定ET-1浓度,肺组织测定ET-1免疫组化。结果 照射后肺组织炎症和肺水肿进行性加重;照射后5,15,30,60dS组血浆ET-1水平较N组进行性升高(P<0.05),L组15,30,60d血浆ET-1水平降低(P<0.05),其余各组差异无显著性。S组ET-130,60d表达增强(P<0.05)。W组30d抑制ET-1表达(P<0.01),L组15d抑制ET-1在肺上皮细胞、巨噬细胞,炎症细胞的表达(P<0.01),30,60d时所有表达部位都出现抑制(P<0.05)。D组60dET-1表达减弱。结论 血浆ET-1水平的变化能反映放射性肺损伤的严重程度,维甲酸、氟伐他汀能抑制肺组织ET-1的表达,降低血浆ET-1水平,可能是维甲酸、氟伐他汀治疗放射性肺损伤的一个作用机理。 |
| 英文摘要: |
| Objective To explore the changes of endothelin-1 in plasma and lung tissues,and the curative effect of retinoic acid as well as fluvastatin in radiation lung injury. Methods Altogether 100 female SD rats were divided randomly into· groups:the control group (N),irradiation alone group(S),retinoic acid treatment group(W),retinoic acid and fluvastatin treatment group(L),and dexamethasone treatment group(D).Each group had 20 rats.Rats of the latter four groups were irradiated with a linear accelaretor at a dose of 20 Gy thoracically.Retinoic acid was gastrogavaged at a dose of 20 mg·kg-1·d-1 in group W on the second day after irradiation,Retinoic acid and fluvastatin were gastrogavaged each at a dose of 10 mg·kg-1·d-1 in group L.Dexamethasone was gastrogavaged at a dose of 3.33 mg·kg-1·d-1 in group D.The contents of endothelin-1 (ET-1) in plasma were measured on days 5,15,30 and 60.ET-1 was also determined in lung tissue by immunohistochemitry. Results Pulmonary edema and pneumonia aggravated gradually in group S and alleviated in group W and group L.ET-1 increased in group L significantly in plasma beginning from day· ( P< 0.05),and decreased on days 15,30,and 60 ( P<0.05).The expression of ET-1 in lung tissues became stronger in groups on days 30 and 60 ( P< 0.05).Retinoic acid could inhibit the expression of ET-1 on day 30 ( P< 0.01),Retinoic acid together with fluvastatin could inhibit the expression of ET-1 in the epithelial cells,macrophages and inflammatory cells on day 15 ( P< 0.05),and all expression sites were inhibited on days 30 and 60 ( P< 0.05).The expression of ET-1 was dim in group D on day 60. Conclusions ET-1 could reflect the degree of radiation lung injury.Retinoic and fluvastatin could inhibit expression of ET-1 in the lung tissue,and they could decrease ET-1 content in plasma significantly.This might be one of their mechanisms in treatment of radiation lung injury. |
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