| 吕秋军,温利青,张敏,等.白藜芦醇的辐射防护及其分子机理的研究[J].中华放射医学与防护杂志,2004,24(1):21-22.LÜ Qiu-jun,WEN Li-qing,ZHANG Min,et al.Study on radiation protective effect of resveratrol and its molecular mechanism[J].Chin J Radiol Med Prot,2004,24(1):21-22 |
| 白藜芦醇的辐射防护及其分子机理的研究 |
| Study on radiation protective effect of resveratrol and its molecular mechanism |
| 投稿时间：2003-03-18 |
| DOI： |
| 中文关键词: 白藜芦醇 辐射 小鼠 凋亡 |
| 英文关键词:Resveratrol Radiation Mouse Apoptosis |
| 基金项目:总后“十五”指令性基金资助项目(01L022) |
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| 中文摘要: |
| 目的 研究白藜芦醇(RES)对受照射小鼠的辐射防护作用及其分子作用机理。方法 采用60Coγ射线照射小鼠模型,观察小鼠30d存活和死亡动物存活时间。用原位末端标记法观察受照射小鼠脾细胞凋亡,流式细胞仪检测脾细胞凋亡率和凋亡相关蛋白的表达水平,同时检测脾细胞内凋亡相关酶活性大小。结果 照射前给予RES能明显提高受照射小鼠的存活率,延长死亡动物存活时间。RES能明显抑制受照射小鼠脾细胞凋亡,提高Bcl2表达水平,对于Fas表达无明显影响,同时脾细胞内的Caspase3和Caspase8活性明显升高。结论 RES具有明显的辐射防护作用,其作用机理与抑制辐射敏感细胞的细胞凋亡有关。 |
| 英文摘要: |
| Objective To investigate radiation-protective effect of resveratrol and its molecular mechanism. Methods Kunming mice were ig administered with resveratrol before 60Coγ-irradiation.Thirty-day survival rate and the average life span of dead mice post-irradiation were observed.The apoptosis of spleen from irradiatied mice was detected by FACS and in situ termianl labeling method.The effect of resveratrol on the activities of Caspase-3 and Caspase-8,and the expression levels of Bcl-2 and Fas were examined. Results Administration with resveratrol resulted in increases of 30-day survival rate and prolongation of average life span of the dead mice.Aoptotic rate of spleen cells decreased,expression level of bcl-2 increased,the expression of Fas did not change,and the activities of Caspase-3 and Caspase-8 increased in spleen cells of irradiation groups. Conclusion The results indicate that resveratrol has radiation-protective effect and its mechanism might be related with its suppression of apoptosis of radiation-sensitive cells. |
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