| 刘耀文,罗庆良,董波,郭德煌,熊国林,郝静,赵蓓蕾,葛平.rhIL-11对急性放射病小鼠骨髓细胞凋亡的影响[J].中华放射医学与防护杂志,2004,24(1):18-20 |
| rhIL-11对急性放射病小鼠骨髓细胞凋亡的影响 |
| Effects of rhIL-11 on apoptosis of bone marrow cells in mice irradiated with 5.5 Gy 60Co γ-rays |
| 投稿时间：2003-03-07 |
| DOI： |
| 中文关键词: 辐射损伤 rhIL-11 造血细胞 凋亡 |
| 英文关键词:Radiation injury rhIL-11 Hematopoietic cells Apoptosis |
| 基金项目:全军“十五”指令性基金资助项目(01L019) |
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| 中文摘要: |
| 目的 研究rhIL11对急性辐射损伤后骨髓造血细胞凋亡的影响。方法 应用原位末端标记、流式细胞术及琼脂糖凝胶电泳法分别在照射后不同时间观察了rhIL11对55Gy60Coγ射线全身照射小鼠骨髓造血细胞凋亡的影响。应用免疫组织化学法观察了各组小鼠骨髓细胞Bcl2和P53蛋白的表达变化。结果 ①照射前应用rhIL11可使照射后6h小鼠骨髓造血细胞凋亡率下降,而照射后给药组照射后12h凋亡率下降更加明显。②照射后3d给药组P53蛋白表达均较照射对照组明显降低(P<0.01),Bcl2蛋白表达则明显升高;照射后7d给药组Bcl2升高仍明显。结论 应用rhIL11可有效抑制照射后小鼠骨髓造血细胞凋亡,有利于造血功能的恢复。rhIL11对细胞凋亡的影响可能与Bcl2和P53等蛋白的调节密切相关。 |
| 英文摘要: |
| Objective To evaluate the effect of rhIL-11 on apoptosis of bone marrow cells of mice irradiated with 5.5 Gy 60Coγ rays. Methods At 6,12,24 hours and on days 3,7 after irradiation,apoptosis of bone marrow cells was studied by means of in situ terminal labeling,flow cytometry and DNA electrophoresis.The expressions of P53 and Bcl-2 of bone marrow cells were evaluated by immunocytochemistry. Results (1)There was a significant descending trend of apoptotic ratio in rhIL-11-treated group 6 and 12 hours after TBI compared with the control group.(2)On day 3,P53 was expressed at a significantly lowere level ( P< 0.01),whereas Bcl-2 at a significantly higher level ( P< 0.01),in rhIL-11-treated groups than those of the placebo controls,respectively.On day 7,expression of Bcl-2 was still higher in rhIL-11-administered-before-TBI group ( P< 0.01). Conclusion rhIL-11 can promote recovery of murine BM cells by protecting them from apoptosis.The effects of rhIL-11 on apoptosis may have close relationship to the modulation of Bcl-2 and P53. |
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