| 胡迎春,项晓琼,徐勤枝,霍艳英,李邦印,段瑞峰,李刚,张开泰,吴德昌.α粒子辐射诱导人肺鳞癌裸鼠转移模型的建立[J].中华放射医学与防护杂志,2004,24(1):16-18 |
| α粒子辐射诱导人肺鳞癌裸鼠转移模型的建立 |
| Establishment of a metastatic model of human lung carcinoma induced by α particles in nude mice |
| 投稿时间：2003-04-24 |
| DOI： |
| 中文关键词: 肺癌 裸鼠转移模型 |
| 英文关键词:Lung cancer Metastatic nude model |
| 基金项目:国家重点基础研究专项资金资助项目(G1998051207) |
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| 中文摘要: |
| 目的 建立α粒子辐射诱导人肺鳞癌裸鼠转移模型。方法 α粒子辐射诱导人肺鳞癌细胞系BERP35T4原代接种裸鼠皮下,后采用组织块接种的方法,在鼠间连续传代3次,计算原代成瘤率及传代成活率,并观察肿瘤的转移情况,病理切片和免疫组织化学鉴定肿瘤的性质和来源。结果 (1)α粒子辐射诱导人肺鳞癌细胞系BERP35T4原代接种成瘤率为44.4%(4/9);(2)原代肿瘤的体表转移率与肺转移率均为25%(1/4);(3)病理切片鉴定为低分化鳞状上皮细胞癌;(4)免疫组织化学显示CK3和EMA表达阳性。结论 采用组织块接种的方法,在α粒子辐射诱导人肺鳞癌细胞系BERP35T4的基础上,经过裸鼠体内连续传代3次,成功地建立了人类肺癌裸鼠转移模型。 |
| 英文摘要: |
| Objective To establish a metastatic model of human lung carcinoma induced by α particles in nude mice. Methods A total of 9 BALB/C nude mice (5-6 weeks old) was inoculated subcutaneously (SC) with human tumor cell line BERP35T4.The tumor nodules developed from the nude mice were passaged for 3 times in nude mice via orthotopic implantation of histologically intact tissues. Results (1)Four of 9 nude mice developed (44.4%)neoplasm at the initial inoculation site.(2)one of four nude mice with neoplasm (25%) presented subcutaneous and lung metastasis.(3)Tumor tissue isolated from nude mice was characterized as squamous epithelial carcinoma with low differentiation.(4)CK3 and EMA staining were positive in cancer cells. Conclusion {A metastatic } model of human lung carcinoma induced by α particles in nude mice has been established via orthotopic implantation of histologically intact tissues. |
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