中华放射医学与防护杂志

刘青杰,陈德清,JulieR.Korenberg,陈晓宁,沈岩.M-FISH技术检测辐射诱导染色体易位和双着丝粒畸变[J].中华放射医学与防护杂志,2004,24(1):13-15

M-FISH技术检测辐射诱导染色体易位和双着丝粒畸变

Radiation-induced translocation and dicentric chromosome aberrations in lymphocytes of umbilical cord blood analyzed by multicolor FISH method

投稿时间：2003-05-20

DOI：

中文关键词: 多色荧光原位杂交易位双着丝粒理论值

英文关键词:Multicolor FISH Translocation Dicentric Predicted value

基金项目:国家863计划项目(2003AA221081);卫生部科学研究基金资助项目(98246);美国能源部基金资助项目[DEFC0396ER62294、DEFG0301ER6326(JRK)]

作者	单位
刘青杰	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所国家人类基因组北方研究中心
陈德清	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所
JulieR.Korenberg	Cedars-Sinai Medical Center, UCLA, USA
陈晓宁	Cedars-Sinai Medical Center, UCLA, USA
沈岩	国家人类基因组北方研究中心

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中文摘要:

目的探讨用多色荧光原位杂交(MFISH)技术检测的易位和双着丝粒染色体畸变的差异。方法用1,2,3,7,8,9,14和15号染色体端粒和着丝粒特异性探针的MFISH方法,分析⁶⁰Coγ射线离体照射的脐带血淋巴细胞染色体易位和双着丝粒畸变。结果 (1)用MFISH方法分析⁶⁰Coγ射线诱导的易位和双着丝粒染色体畸变的剂量效应曲线,均符合线性二次剂量效应模式;易位与双着丝粒的比值在大多数剂量水平不等于1。(2)细胞中无非稳定性畸变的完全相互易位的比例随着吸收剂量的增加而降低。(3)对大多数被标记染色体,3.00Gyγ射线照射诱发的染色体畸变观察值与理论值无差别;9号染色体畸变(易位和双着丝粒)观察值显著高于理论值(P<0.05或P<0.01),15号染色体易位观察值显著低于理论值(P<0.01)。结论电离辐射诱导的染色体易位率不等于双着丝粒畸变率;对于大多数染色体,辐射诱发染色体易位率和双着丝粒畸变率符合随机性规律。

英文摘要:

Objective To explore the differences between radiation-induced translocation and dicentric chromosome aberrations detected by multicolor fluorescence in situ hybridization (M-FISH) method. Methods The translocation and dicentric chromosome aberrations in lymphocytes of umbilical cord blood induced by different doses of ⁶⁰Cogamma rays were analyzed by M-FISH.The specific telomeres and centromeres BAC clones of chromosomes 1,2,3,7,8,9,14 and 15 were used for M-FISH probes. Results (1)The dose-response curves of translocation and dicentric aberrations with absorbed dose levels accorded to linear-quadratic dose-response model.The translocation frequency was not equal to the dicentric frequency at most dose levels;(2)The proportion of cells with complete translocation but without unstable chromosome aberration decreased with increasing absorbed dose;(3)The observed values and the predicted values of chromosome aberrations,induced by 3 Gy gamma radiation,were not significantly different in most of the labeled chromosomes,but chromosome 9 had observed values significantly higher than predicted values ( P< 0.05 or P<0.01),and chromosome 15 vice versa ( P< 0.01). Conclusion The frequency of chromosome translocation induced by ionizing radiation is not equal to that of dicentric aberration.The frequencies of translocation and dicentric chromosome aberrations induced by ionizing radiation are at random for most chromosomes.

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