| 黄越承,蔡建明,韩玲,高福,崔建国,高建国.γ射线诱发的小鼠急性淋巴细胞白血病中MDM2的表达研究[J].中华放射医学与防护杂志,2004,24(1):6-9 |
| γ射线诱发的小鼠急性淋巴细胞白血病中MDM2的表达研究 |
| Expression of MDM2 in an acute lymphocytic leukemia mice model induced by γ-radiation |
| 投稿时间：2003-06-27 |
| DOI： |
| 中文关键词: MDM2 白血病 Westernblot 原位杂交 |
| 英文关键词:MDM2 Leukemia Western blot In situ hybridization |
| 基金项目:国家自然科学基金资助项目(30170282) |
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| 中文摘要: |
| 目的 观察MDM2在γ射线诱发的小鼠淋巴细胞白血病组织细胞中的表达变化,探讨MDM2在辐射致癌中的作用及可能的分子机理。方法 用γ射线照射BALBc小鼠诱发白血病发生,建立辐射致癌动物模型;分别应用Westernblot和原位杂交技术,从蛋白水平和mRNA水平检测小鼠胸腺和骨髓组织中MDM2的表达情况;运用免疫沉淀技术(IP)检测MDM2蛋白的磷酸化水平;PCRSSCP及银染技术用于检测基因突变。结果 癌变组和辐射未癌变组胸腺细胞骨髓细胞MDM2蛋白表达水平都高于对照组(P<0.05);而癌变组和辐射未癌变组之间MDM2蛋白水平差异无显著性(P>0.05)。γ射线照射后早期辐射组骨髓MDM2蛋白表达水平也明显高于对照组。原位杂交结果显示:癌变组织中MDM2阳性反应和阳性率均明显高于对照组。在癌变组组织中发现有MDM2磷酸化比其他组都明显升高(P<0.05)。辐射组和对照组均未检测到基因突变。结论 MDM2可能参与γ射线诱发的小鼠淋巴细胞白血病的发生和发展过程,作用机理可能与过表达及高磷酸化所致活性增强有关。MDM2的基因突变在辐射致小鼠白血病发生不起主要作用。 |
| 英文摘要: |
| Objective To investigate the role of the MDM2 in the process of carcinogenesis induced by γ-rays and its molecular mechanisms. Methods Animal model of radiation-induced leukemia was established by γ-irradiation.According to the histological and morphological results,mice tissues were divided into three groups:cancerization group,incancerization group and control group.Expression of MDM2 protein and mRNA in thymus/bone marrow was detected with Western blot and in situ hybridization (ISH),respectively.We also examined the protein phosphorylation level of MDM2 protein by immunoprecipitation (IP).PCR-SSCP was performed to detect gene mutation. Results A mice leukemia model was successfully established as verified by pathological findings and confirmed by transplantation test in nude mice.The protein expression in thymus/bone marrow in irradiation groups was significantly higher than that in controls ( P<0.05).But the expression level was not statistically different between the cancerization group and the incancerization group.The MDM2 was found to be hyperphosphorylated in the cancerization group as compared with other groups.No gene mutation was detected by SSCP/silver-staining assay in the tumor samples. Conclusion MDM2 may be involved in the development and progression of leukemia induced by γ-irradiation.The over-expression but not gene mutation may be responsible for malignant transformation induced by radiation.Phosphorylation is at least partly attributed to activation of MDM2. |
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