中华放射医学与防护杂志

何东初,王晓红,何辉,等.地甘口服液对辐射损伤小鼠细胞周期及凋亡相关基因的影响[J].中华放射医学与防护杂志,2003,23(6):432-434.HE Dongchu,WANG Xiaohong,HE Hui,et al.Effect of Digan oral liquid on expression of cell cycle progression and apopto sis-related genes in mice with radiation lesions[J].Chin J Radiol Med Prot,2003,23(6):432-434

地甘口服液对辐射损伤小鼠细胞周期及凋亡相关基因的影响

Effect of Digan oral liquid on expression of cell cycle progression and apopto sis-related genes in mice with radiation lesions

投稿时间：2003-01-04

DOI：

中文关键词: 地甘口服液辐射损伤凋亡基因表达

英文关键词:Digan oral liquid Radiation injury Apoptosis Gene expression

基金项目:湖北省自然科学基金资助项目(97J080)

作者	单位
何东初	430070 武汉, 广州军区武汉总医院中西医结合科
王晓红	430070 武汉, 广州军区武汉总医院中西医结合科
何辉	430070 武汉, 广州军区武汉总医院中西医结合科
陈如泉	湖北省中医学院附院

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中文摘要:

目的探讨中药复方地甘口服液对辐射损伤小鼠骨髓细胞周期素基因(cyclinD₁)及凋亡相关基因(bcl-2和bax)mRNA表达的作用。方法建立辐射损伤模型(一次性用直线加速器给予6.0Gy的X射线照射),喂饲小剂量100%和大剂量200%的地甘口服液每次0.2ml10g小鼠,每日2次。第9天用原位杂交法检测小鼠骨髓单个核细胞cyclinD₁、bcl-2和baxmRNA的表达。结果地甘口服液组与对照组比骨髓细胞促凋亡基因baxmRNA表达显著减少(P<0.01),而cyclinD₁和bcl-2m-RNA的表达显著增强(P<0.01)。结论中药复方地甘口服液抗辐射损伤可能是通过上调细胞周期素基因cyclinD₁和bcl-2的表达及降低baxmRNA的表达,从而促进造血细胞的增生和提高机体免疫力,达到保护机体的目的,为临床用药提供理论指导。

英文摘要:

Objective To study the effect of Digan oral liquid (DGOL) on expression of cell cycle prog ression(cyclinD₁)- and apoptosis(bcl-2 and bax)- related genes in bone marr ow of mice with radiation lesions. Methods After the radiation lesion model(given 6 0 Gy X-rays on ce by a linear accelerator) was established,the animals were fed with 0 2 ml/10 g of either small dose (100%) or large dose (200%) DGOL twice a day for 8 days. On the ninth day cyclinD₁,bcl-2 and bax mRNA expression levels in marrow MNC of model mice were measured by in situ hybridization. Results In the DGOL group,the bax mRNA expression was significantly decreased (P<0.01),but cyclinD₁ and bcl-2 mRNA were significantly increased (P<0.01) t han those in the control group. Conclusion DGOL can restrain radiation lesion due to its enhancing effects on cyclinD₁ an d bcl-2 mRNA expression,and inhibitory effect on bax mRNA expression.In this wa y,it prevents the blood cells from injury,promotes immunity,and protects the org anism.This result provides a theoretical basis for clinical application of Diga noral liquid.

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