| 何东初,王晓红,何辉,陈如泉.地甘口服液对辐射损伤小鼠细胞周期及凋亡相关基因的影响[J].中华放射医学与防护杂志,2003,23(6):432-434 |
| 地甘口服液对辐射损伤小鼠细胞周期及凋亡相关基因的影响 |
| Effect of Digan oral liquid on expression of cell cycle progression and apopto sis-related genes in mice with radiation lesions |
| 投稿时间：2003-01-04 |
| DOI： |
| 中文关键词: 地甘口服液 辐射损伤 凋亡 基因表达 |
| 英文关键词:Digan oral liquid Radiation injury Apoptosis Gene expression |
| 基金项目:湖北省自然科学基金资助项目(97J080) |
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| 中文摘要: |
| 目的 探讨中药复方地甘口服液对辐射损伤小鼠骨髓细胞周期素基因(cyclinD1)及凋亡相关基因(bcl-2和bax)mRNA表达的作用。方法 建立辐射损伤模型(一次性用直线加速器给予6.0Gy的X射线照射),喂饲小剂量100%和大剂量200%的地甘口服液每次0.2ml10g小鼠,每日2次。第9天用原位杂交法检测小鼠骨髓单个核细胞cyclinD1、bcl-2和baxmRNA的表达。结果 地甘口服液组与对照组比骨髓细胞促凋亡基因baxmRNA表达显著减少(P<0.01),而cyclinD1和bcl-2m-RNA的表达显著增强(P<0.01)。结论 中药复方地甘口服液抗辐射损伤可能是通过上调细胞周期素基因cyclinD1和bcl-2的表达及降低baxmRNA的表达,从而促进造血细胞的增生和提高机体免疫力,达到保护机体的目的,为临床用药提供理论指导。 |
| 英文摘要: |
| Objective To study the effect of Digan oral liquid (DGOL) on expression of cell cycle prog ression(cyclinD1)- and apoptosis(bcl-2 and bax)- related genes in bone marr ow of mice with radiation lesions. Methods After the radiation lesion model(given 6 0 Gy X-rays on ce by a linear accelerator) was established,the animals were fed with 0 2 ml/10 g of either small dose (100%) or large dose (200%) DGOL twice a day for 8 days. On the ninth day cyclinD1,bcl-2 and bax mRNA expression levels in marrow MNC of model mice were measured by in situ hybridization. Results In the DGOL group,the bax mRNA expression was significantly decreased (P<0.01),but cyclinD1 and bcl-2 mRNA were significantly increased (P<0.01) t han those in the control group. Conclusion DGOL can restrain radiation lesion due to its enhancing effects on cyclinD1 an d bcl-2 mRNA expression,and inhibitory effect on bax mRNA expression.In this wa y,it prevents the blood cells from injury,promotes immunity,and protects the org anism.This result provides a theoretical basis for clinical application of Diga noral liquid. |
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