| 郭卫东,庄永志,程玉龙,贾廷珍.低剂量电离辐射对7721细胞细胞周期及p53、Ku70和Ku80表达的影响[J].中华放射医学与防护杂志,2003,23(6):421-423 |
| 低剂量电离辐射对7721细胞细胞周期及p53、Ku70和Ku80表达的影响 |
| The effect of ionizing radiation on expression of DNA repair genes and cell cycle progression of human liver cancer cells |
| 投稿时间：2002-09-16 |
| DOI： |
| 中文关键词: 低剂量电离辐射 细胞周期 7721细胞 DNA修复基因 |
| 英文关键词:Low dose radiation Cell cycle progression 7721 cell DNA repair gene |
| 基金项目:国家自然科学基金资助项目(39770238) |
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| 中文摘要: |
| 目的 观察电离辐射对7721细胞(人肝癌细胞)细胞周期和p53、Ku70和Ku80基因表达的影响。方法 以人肝癌细胞株7721为研究对象,通过克隆形成实验拟合出7721细胞的剂量存活曲线;用流式细胞技术检测75mGyX射线照射后7721细胞周期变化;用原位杂交方法检测7721细胞p53、Ku70和Ku80基因在75mGyX射线照射前、后的表达情况。结果 与对照组相比,75mGyX射线照射后0.5～6h,7721细胞S期延长(P<0.05)。p53、Ku70和Ku80基因的表达照射前与照射后比较差异无显著性。结论 7721细胞在75mGyX射线照射后,未出现G1期阻滞,p53、Ku70和Ku80基因在照射前、后表达无明显变化,是由于这些基因自身存在缺陷或激活机制存在缺陷所致。 |
| 英文摘要: |
| Objective To investigate the effect of ionizing radiation on the expression of p53,Ku70 an d Ku80 genes and the cell cycle progression of 7721 cell. Methods Dose-survival curve of 7721 cells was figured by colony-forming assay.7721 cells were irradiated with X-rays at the dose of 75 mGy a nd the cells were collected at different times after irradiation.Cell cycle was a nalyzed by FACS and the expressions of p53,Ku70 and Ku80 genes were detected by in situ hybridization. Results The S phase of 7721 cells was prolonged after 75 mGy X-r ay irradiation compared with that of the control group (P<0.01).There was n o change in the expression of p53,Ku70 and Ku80 genes after irradiation. Conclusion There is no G1 arrest effect of radiation at low dose on 7721 cells.The expressions of p53,Ku70 and Ku80 are not influenced by radiat ion. |
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