| 汤屹,刘文励,周剑锋,高庆蕾,吴剑宏.60Coγ射线照射后肿瘤细胞株的细胞周期阻滞变化[J].中华放射医学与防护杂志,2003,23(6):418-420 |
| 60Coγ射线照射后肿瘤细胞株的细胞周期阻滞变化 |
| Change of cell cycle arrest of tumor cell lines after 60Coγ-irradia tion |
| 投稿时间：2002-12-18 |
| DOI： |
| 中文关键词: 肿瘤细胞株 放射敏感性 细胞周期阻滞 |
| 英文关键词:Tumor cell lines Radiation sensitivity Cell cycle arrest |
| 基金项目:国家自然科学基金资助项目(39800149) |
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| 摘要点击次数: 2743 |
| 全文下载次数: 1991 |
| 中文摘要: |
| 目的 观察正常人外周血单个核细胞(peripheral blood mononuclear cells,PBMNCs)及几种肿瘤细胞株经60Co源照射后其细胞周期阻滞现象的动态变化。方法 用60Co源分别以6,10,15Gy的吸收剂量照射正常人PBMNCs及HL-60、K562、SiHA和113肿瘤细胞株。并于照射后6,12,24,48及60h以流式细胞仪测量其细胞周期变化。结果 正常终末细胞的细胞周期基本处于停滞状态,95%细胞均处于G1期,当其接受照射后依然表现周期停滞状态;除113细胞表现G1期阻滞外,其他肿瘤细胞在受到照射后,均表现G2M期阻滞;HL-60放射敏感性较SiHA强。结论 不同种类细胞对放射线表现的细胞周期阻滞现象不同,其放射敏感性也存在差异。 |
| 英文摘要: |
| Objective To observe the cell cycle arrest changes in peripheral blood mononuclear cells ( PBMNCs) of normal persons and several kinds of tumor cell lines after 60Coγ-irradiation. Methods PBMNCs of normal persons,HL-60,K562,SiHA and 113 tumor cell lines were irradiat ed with 60Coγ-rays at the absorbed doses of 6,10,15 Gy.Cell cycles changes were checked 6,12,24,48 and 60 h after the irradiation. Results A stasis state was observed in normal person PBMNCs, 95 p ercents of which were in G1 phase,and they still remained stasis after the irr adiation.Except the 113 cell line manifesting G1 phase arrest,all other tumor cell lines showed G2/M phase arrest after irradiation.The radiation sensitivit y of HL-60 was higher than that of SiHA cell line. Conclusion Different cell lines have different cell cycle arrest r eaction to radiation and their radiation sensitivity are also different. |
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