中华放射医学与防护杂志

金顺子,何淑杰,刘树铮.低剂量电离辐射诱导转录因子NF-κB活化及其信号通路的实验研究[J].中华放射医学与防护杂志,2003,23(6):408-410

低剂量电离辐射诱导转录因子NF-κB活化及其信号通路的实验研究

Experimental studies on the activation of NF-κB and its signal pathway induced by low dose radiation

投稿时间：2003-03-03

DOI：

中文关键词: 低剂量辐射信号传导通路 NF-κB IκB Calphostin C

英文关键词:Low dose radiation Signal pathway NF-κB I κB Calphostin C

基金项目:

作者	单位
金顺子	130021 长春, 吉林大学公共卫生学院卫生部放射生物学重点实验室
何淑杰	130021 长春, 吉林大学公共卫生学院卫生部放射生物学重点实验室
刘树铮	130021 长春, 吉林大学公共卫生学院卫生部放射生物学重点实验室

摘要点击次数: 2463

全文下载次数: 1569

中文摘要:

目的研究低剂量电离辐射对转录因子NF-κBDNA结合活性的影响,探讨NF-κB结合活性信号传导通路的机理。方法采用凝胶电泳移动变化分析及免疫组织化学的方法检测了X射线照射后EL4细胞NF-κBDNA结合活性的时程变化及p65核转位、IκBα水平的变化,同时用脂质体介导的瞬时转染法将空质粒和NF-κB荧光素酶表达质粒转染到NIH3T3细胞内,并用PKC信号通路阻断剂CalphostinC处理,检测受照后其荧光素酶的表达变化。结果 0.075GyX射线照射可诱导NF-κB、p50/p65和p50/p50活性增强,而前者活性增强更明显。这种转录激活能力的增强,涉及到辐射诱导的p65核转位和IκBα水平的变化,表现为受照后p65亚基核阳性率明显升高,而IκBα水平的变化正相反,在照后4h分别达到峰值和低谷。而且PCK信号通路阻断剂CalphostinC可降低0.075Gy照射对NF-κB的活化效应。结论低水平照射诱导NF-κB的迅速活化,而且可能通过PKC通路,进而调节细胞因子等特异基因的表达,促使免疫功能增强,这可能是低剂量辐射兴奋效应的机理之一。

英文摘要:

Objective To observe the effect of low dose radiation (LDR) on the activation of NF-κB and the possible mechanism of its signal tra nsduction. Methods Electrophoretic mobility shift assay (EMSA) and immunohistochemistry were used to detect the changes of DNA-binding activity o f NF-κB and the nuclear translocation of p65 in EL-4 cells,respectively.Meanw hile,LipofectAMINE-mediated transient transfection was used to carry vacant pla smid and NF-κB-luciferase-containing plasmid into NIH3T3 cells,with or witho ut the addition of PKC-specific blocking agent Calphostin C,to detect the expre ssion of luciferase after irradiation. Results X-rays of 0.075 Gy increased the activities of p50/p65 a nd p50/p50 dimers,especially the former.The translocation of p65 into nuclei inc reased and IκBα exhibited the opposite changes.Calphostin C reversed the effec t of 0.075 Gy radiation on the activity of NF-κB. Conclusion The activation of NF-κB induced by LDR might be effec ted via the PKC pathway,resulting in the facilitation of the expression of cytok ines and up-regulation of immune functions,which might be involved in the mecha nisms of excitatory effects of LDR.

HTML 查看全文查看/发表评论下载PDF阅读器

关闭