| 鞠桂芝,王晓梅,付士波,刘树铮.电离辐射对胸腺细胞及脾细胞p16、CyclinD1、CDK4蛋白表达的影响[J].中华放射医学与防护杂志,2003,23(6):399-401 |
| 电离辐射对胸腺细胞及脾细胞p16、CyclinD1、CDK4蛋白表达的影响 |
| Effect of ionizing radiation on protein expressions of p16,Cyclin D1 and CDK4 in thymocytes and splenocytes of mice |
| 投稿时间：2003-03-03 |
| DOI： |
| 中文关键词: 电离辐射 p16 CyclinD CDK4 |
| 英文关键词:Ionizing radiation p16 Cyclin D CDK4 |
| 基金项目:国家自然科学基金资助项目(30270346) |
|
| 摘要点击次数: 2652 |
| 全文下载次数: 1634 |
| 中文摘要: |
| 目的 研究电离辐射对小鼠胸腺细胞及脾细胞中p16、CyclinD1及CDK4蛋白表达的影响。方法 采用单克隆抗体免疫荧光标记及流式细胞术检测蛋白表达的变化。结果 时效实验证实,2.0GyX射线全身照射后8,24及48h,胸腺细胞p16蛋白表达显著增高(分别P<0.05、P<0.01及P<0.05);照射后24h,脾细胞p16蛋白表达显著增高(P<0.05)。然而,照射后8～24h,胸腺细胞CDK4蛋白表达显著降低(P<0.05～P<0.01);照射后8～72h,脾细胞CDK4蛋白表达显著降低(P<0.05～P<0.01)。量效实验证实,1.0,2.0及4.0Gy全身照射后24h,胸腺细胞及脾细胞p16蛋白表达均显著增高(P<0.05～P<0.01)。然而,2.0Gy照射后胸腺细胞CDK4蛋白表达显著降低(P<0.05);0.5～6.0Gy照射后脾细胞CDK4蛋白表达显著降低(P<0.05～P<0.01)。研究还发现,胸腺细胞及脾细胞中CyclinD1蛋白表达于照射后均明显降低。结论 电离辐射可诱导胸腺细胞及脾细胞中p16蛋白表达增高。p16-CyclinD1/CDK4通路可能在X射线诱导胸腺细胞G1期阻滞中起重要作用。 |
| 英文摘要: |
| Objective To investigate the effect of ionizing radia tion on the expressions of p16,CyclinD1 and CDK4 in thymocytes and splenocytes o f mice. Methods Fluorescent staining and flow cytometric analysis were employed for measurement of protein expressions. Results It was found in time course experiments that the expression of p16 protein incre ased significantly at 8,24 and 48 h in thymocytes (P<0.05,P<0.01 and P<0.05, respectively) and at 24 h in splenocytes (P<0.05) after whol e-body irradiation (WBI) with 2.0 Gy X-rays.However,the expression of CDK4 pr otein decreased significantly from 8 to 24 h in thymocytes (P<0.05-P<0.01) and from 8 to 72 h in splenocytes (P<0.05-P<0.01).In dose-effe ct experiments,it was found that the expression of p16 protein in thymocytes and splenocytes increased significantly at 24 h after WBI with the doses of 1.0,2.0 and 4.0 Gy(P<0.05-P<0.01),whereas the expression of CDK4 protein decreased significantly with the dose of 2.0 Gy for thymocytes (P<0.05) a nd with the doses from 0.5 to 6.0 Gy for splenocytes (P<0.05-P<0.01 ).The expression of CyclinD1 protein decreased markedly in both thymocytes and s plenocytes after exposure. Conclusion The results indicate that th e expression of p16 protein in thymocytes and splenocytes could be induced by io nizing radiation,and the p16-CyclinD1/CDK4 pathway might play an important role for G1 arrest of thymocytes induced by X-rays. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|