| 王长振,丛建波,先宏,孙存普,吴可.电磁脉冲造成神经细胞氧化损伤效应研究[J].中华放射医学与防护杂志,2003,23(5):361-364 |
| 电磁脉冲造成神经细胞氧化损伤效应研究 |
| Studies on oxidative effects induced by electromagnetic pulse on neural cells |
| 投稿时间：2002-10-15 |
| DOI： |
| 中文关键词: 电磁脉冲 神经细胞 自由基 氧化应激 |
| 英文关键词:Electromagnetic pulse Neural cells Free radicals Oxidative stress |
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| 中文摘要: |
| 电磁脉冲(EMP)作为一种非电离辐射,生物效应广泛,从自由基生物学角度研究其对神经细胞的辐射损伤效应具有一定的实际意义。方法 采用自旋捕捉的方法检测自由基的变化,TBA法检测脂质过氧化产物丙二醛(MDA),DTNB法检测谷胱甘肽过氧化物酶(GSH-px)的活性,MTT法检测细胞存活率,流式细胞术检测细胞内Ca2+浓度。结果 电磁脉冲作用大鼠大脑皮层细胞和海马细胞后,脂氧自由基水平升高。MDA水平升高,大脑皮层细胞对照组MDA水平为(6.18±0.29)nmol/mg,EMP作用组为(8.43±0.01)nmol/mg(P<0.01);海马细胞对照组MDA水平为(4.38±0.15)nmol/mg,EMP作用组为(4.98±0.39)nmol/mg(P<0.05)。GSH-px活性降低,大脑皮层细胞对照组A值为0.026±0.001,EMP作用组为0.022±0.002(P<0.05),海马细胞对照组A值为0.021±0.002,EMP作用组为0.018±0.001(P<0.05)。电磁脉冲不能直接杀死细胞,其存活率约为95%,当脉冲数达到10次时,细胞内Ca2+浓度开始升高。结论 电磁脉冲提高了神经细胞脂氧自由基水平,脂质过氧化产物MDA水平升高,GSH-px活性降低,预示氧化损伤效应的发生。另外,电磁脉冲不能直接杀死细胞,但可导致细胞内Ca2+浓度升高,增多的Ca2+可能成为诱发细胞凋亡的重要因素之一。 |
| 英文摘要: |
| Objective As a kind of non-ionizing radiation, bio-effects of electromegnetic pulse (EMP) are numerous.It is meaningful to study effects of EMP at the level of free radicals. Methods Using spin-trapping technique,TBA method,DTNB method,MTT method and FCM technique,changes of free radicals,MDA content,GSH-px activity,percentage of living cells and Ca2+ concentration were detected,respectively. Results The content of lipid peroxide free radicals increased after EMP irradiation on hippocampal cells and cerebral cortex cells of Wistar rat.The content of MDA of cerebral cortex cells in the control group and EMP group was (6.18±0.29)nmol/mg and (8.43±0.01)nmol/mg(P<0.01),respectively.MDA of hippocampal cells in the control group and EMP group was (4.38±0.15)nmol/mg and (4.98±0.39)nmol/mg(P<0.05),respectively.The activities of GSH-px decreased significantly.The OD value of cerebral cortex cells of the control group was 0.026±0.001 and EMP group,0.022±0.002(P<0.05).The OD value of hippocampal cells of the control groupwas 0.021±0.002 and EMP group,0.018±0.001(P<0.05).EMP could not kill the cells directly for the percentage of living cells in the EMP group was about 95%.The concentration of Ca2+ in the cells increased after 10 times of EMP irradiation. Conclusions EMP enhanced the level of free radicals in neural cells. The MDA level increased,and the activity of GSH-px decreased.Additionally,EMP could not kill cells directly,but increased concentration of Ca2+ that might induce apoptosis. |
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