中华放射医学与防护杂志

杜遵民,刘传方,张玉昆,等.抗CD49d McAb联合rhG-CSF动员的PBSC救治放射损伤小鼠[J].中华放射医学与防护杂志,2003,23(5):334-336.DU Zunmin,LIU Chuanfang,ZHANG Yukun,et al.Experimental study on rescue effect of peripheral blood stem cells mobilized by combination of anti-CD49d monoclonal antibody with rhG-CSF in lethally irradiated mice[J].Chin J Radiol Med Prot,2003,23(5):334-336

抗CD49d McAb联合rhG-CSF动员的PBSC救治放射损伤小鼠

Experimental study on rescue effect of peripheral blood stem cells mobilized by combination of anti-CD49d monoclonal antibody with rhG-CSF in lethally irradiated mice

投稿时间：2002-12-30

DOI：

中文关键词: 抗CD49d单克隆抗体粒细胞集落刺激因子动员剂造血干细胞放射损伤

英文关键词:Anti-CD49d monoclonal antibody Granulocyte-colony stimulating factor Mobilization Hematopoietic stem cell Radiation injury

基金项目:济南军区“十五”重点科研项目(02Z49)

作者	单位
杜遵民	271000 山东泰安, 解放军第八八医院血液科
刘传方	山东大学齐鲁医院血液科
张玉昆	山东省医学科学院基础医学研究所
唐天华	山东省医学科学院基础医学研究所
张立成	271000 山东泰安, 解放军第八八医院血液科
任海全	山东省医学科学院基础医学研究所
孙军山	271000 山东泰安, 解放军第八八医院血液科

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中文摘要:

目的观察抗CD49d单克隆抗体(McAb)联用重组人粒细胞集落刺激因子(rhG-CSF)动员的外周血干细胞(PBSC)救治放射损伤小鼠的效果。方法 85Gy⁶⁰Coγ射线照射的BALBc小鼠,分别接受经rhG-CSF(1组)、抗CD49dMcAb(2组)、rhG-CSF联合抗CD49dMcAb(3组)动员的PBSC移植。并以生理盐水组为对照,观察受体小鼠的4周存活率、外周血白细胞(WBC)、骨髓有核细胞(BMNC),粒细胞巨噬细胞集落形成单位(CFU-GM)及脾集落形成单位(CFU-S)等指标。结果实验3组小鼠的4周存活率、WBC、BMNC,CFU-GM、CFU-S数均明显高于对照组、1组和2组,差异具有显著性或非常显著性(P<0.05或P<0.01)。结论抗CD49dMcAb联合rhG-CSF使用能协同动员小鼠PBSC,并能成功救治放射损伤小鼠。

英文摘要:

Objective To study the rescue effects of peripheral blood stem cell (PBSC) mobilized by anti-CD49d monoclonal antibody (McAb) combined with recombinant human granulocyte colony-stimulating factor (rhG-CSF) on lethally irradiated mice. Methods BALB/c mice received 8.5 Gy total body irradiation were transplanted with either NS (control group),PBSCs from rhG-CSF-mobilized mice (experimental group 1),PBSCs from anti-CD49d McAb-mobilized mice (experimental group 2),or PBSCs from anti-CD49d McAb and rhG-CSF-combinatively mobilized mice (experimental group 3).The white blood cells count,4-week survival rate,and numbers of bone marrow nucleated cells (BMNC),granulocyte-macrophage colony-forming unit (CFU-GM),colony-forming unit-spleen (CFU-S) were observed. Results The survival rate and WBC,BMNC,CFU-GM,CFU-S counts were all significantly higher in experimental group 3 than those in the control group,experimental group 1,and experimental group 2 (P<0.01 orP<0.05). Conclusions Anti-CD49d McAb and rhG-CSF may synergetically mobilize PBSC in mice.PBSCs mobilized by combination of anti-CD49d McAb and rhG-CSF could effectively rescue lethally irradiated mice.

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