中华放射医学与防护杂志

潘晓燕,何继亮,张美辨,等.低强度微波与4-硝基喹啉氧化物对人淋巴细胞DNA损伤的研究[J].中华放射医学与防护杂志,2003,23(4):301-303.PAN Xiaoyan,HE Jiliang,ZHANG Meibian,et al.Study of synergistic DNA-damaging effects of low-intensity 2450 MHz microwave and 4-nitroquinoline on human lymphocytes in vitro[J].Chin J Radiol Med Prot,2003,23(4):301-303

低强度微波与4-硝基喹啉氧化物对人淋巴细胞DNA损伤的研究

Study of synergistic DNA-damaging effects of low-intensity 2450 MHz microwave and 4-nitroquinoline on human lymphocytes in vitro

投稿时间：2002-10-21

DOI：

中文关键词: 微波 4-硝基喹啉氧化物彗星试验协同效应人外周血淋巴细胞

英文关键词:Microwave 4-NQO Comet assay Synergistic effect Human lymphocytes

基金项目:浙江省自然科学基金资助项目(300434);浙江省科技厅国际合作项目(012104)

作者	单位	E-mail
潘晓燕	310006 杭州, 浙江大学医学院劳动卫生与环境卫生研究所	he-jiliang@hotmail.com
何继亮	310006 杭州, 浙江大学医学院劳动卫生与环境卫生研究所
张美辨	310006 杭州, 浙江大学医学院劳动卫生与环境卫生研究所
金力奋	310006 杭州, 浙江大学医学院劳动卫生与环境卫生研究所

摘要点击次数: 3431

全文下载次数: 2011

中文摘要:

目的研究低强度2450MHz微波(5.0mW/cm²)与4硝基喹啉氧化物(4-NQO)对人淋巴细胞DNA损伤效应的联合作用。方法采用彗星试验来检测人外周血淋巴细胞经微波与4-NQO联合作用后的DNA损伤情况。微波与4-NQO联合暴露方式有3种:微波辐射后4-NQO染毒、微波与4-NQO同时暴露、4-NQO染毒后微波辐射。结果微波辐射组的DNA损伤指标(彗星形状和彗星全长)与对照组比较,差异无显著性(P>0.05)。微波照射后4-NQO染毒组中,50,25μmolL剂量的DNA损伤指标与相应4-NQO组比较差异均有显著性(P<0.05或P<0.01)。微波与4-NQO同时暴露组或4-NQO染毒后微波照射组与相应4-NQO组比较差异无显著性(P>0.05)。结论低强度2450MHz微波不能诱发DNA损伤,当微波辐射先于4-NQO暴露时,微波能增强4-NQO诱发的DNA损伤效应。

英文摘要:

Objective To study the synergistic DNA-damaging effects of low-intensity 2 450 MHz microwave(MW, 5.0 mW/cm²)and 4-nitroquinoline(4-NQO)on human lymphocytes. Methods The DNA damages of human lymphocytes treated with microwave and 4-NQO were detected using the comet assay.Three combination-exposure ways of MW and 4-NQO were used:MW irradiation before 4-NQO exposure(MW+4-NQO), simultaneous exposure to MW and 4-NQO(MW-4-NQO), and MW irradiation after 4-NQO exposure(4-NQO+MW). Results The differences of DNA-damage indexes (comet tail length, comet shape and total comet length)between MW group and control group were not significant (PP>0.05).When the dose of 4-NQO was 50 μmol/L or₂5 μmol/L, the differences of DNA-damage indexes between MW+4-NQO group and corresponding 4-NQO group were significant(PP<0.05 or PP<0.01).The differences of DNA-damage indexes between MW-4-NQO group or 4-NQO+MW group and corresponding 4-NQO group were not significant(PP>0.05). Conclusion Low-intensity 2 450 MHz microwave does not induce DNA-damage directly, but could enhance the DNA-damaging effects induced by 4-NQO when human lymphocytes have been treated with MW irradiation before 4-NQO exposure.

HTML 查看全文查看/发表评论下载PDF阅读器

关闭