| 邢诒刚,唐亚梅,孙颖,等.急性放射性脑损伤鼠模型的建立[J].中华放射医学与防护杂志,2003,23(4):269-270.XING Yigang,TANG Yamei,SUN Ying,et al.Development of a murine model of acute radiation encephalopathy[J].Chin J Radiol Med Prot,2003,23(4):269-270 |
| 急性放射性脑损伤鼠模型的建立 |
| Development of a murine model of acute radiation encephalopathy |
| 投稿时间：2003-01-31 |
| DOI： |
| 中文关键词: 放射损伤 动物模型 脑 |
| 英文关键词:Radiation injury Animal mode Brain |
| 基金项目: |
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| 摘要点击次数: 3795 |
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| 中文摘要: |
| 目的 建立急性放射性脑损伤动物模型。方法 SD大鼠头部接受60Coγ射线照射后。每日观察大鼠摄食、饮水量,自主活动情况及神经系统症状与体征;每周检查照射区皮肤情况及体重变化,末次照射结束后第3,7,14,30天断头取脑行病理组织学检查。结果 放射疗程中及结束后均未出现异常神经系统体征;两组的自主活动、每日摄食、饮水量及体重均无统计学差异;末次照射后约2周时出现照射野轻度脱毛;照射后出现脑组织神经元变性坏死。结论 该模型制作方法能较好地模拟放射性脑损伤的过程,可用于放射性脑损伤的实验研究。 |
| 英文摘要: |
| Objective To develop a murine model of acute radiation encephalopathy. Methods A total of 40 rats were subjected to local γ-irradiation to the brain with the dosage of 7 Gy/d for 6 consecutive days.The amoute of food intake, hairs and skin of irradiated field, body weight, general activities, CNS symptoms and signs were examined and recorded after irradiation.On day 3, 7, 14 and 30, the brain tissue was removed to observe histopathologic changes. Results During the first two days after irradiation, the irradiated rats were agitated, and the amount of food intake decreased from day 2 onwards.No serious skin reaciton to irradiation was observed.Survived rats had normal activities without any abnormal nervous signs.Histopathologic changes showed slight neuronal degeneration, smaller cell body, red-colored cytoplasm, disappearance of Nissl body, vacuolation, typical cell shrinkage, chromatin condensation and nuclear divergence.On the 14th and 30th days, hypochromatism, loose and reticular necrotic foci were found in some samples. Conclusion Our murine model of acute radiation encephalopathy is useful and practical in radiobiologic studies. |
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