| 魏启春,郑树.放射抗拒肺癌细胞亚系D6-R的建立及其细胞周期研究[J].中华放射医学与防护杂志,2003,23(4):267-269.WEI Qichun,ZHENG Shu.Establishment and cell cycle distribution pattern of a radioresistant subline from human lung cancer D6 cell line[J].Chin J Radiol Med Prot,2003,23(4):267-269 |
| 放射抗拒肺癌细胞亚系D6-R的建立及其细胞周期研究 |
| Establishment and cell cycle distribution pattern of a radioresistant subline from human lung cancer D6 cell line |
| 投稿时间：2003-01-22 |
| DOI： |
| 中文关键词: 肺 肿瘤 辐射 放射敏感性 细胞周期 |
| 英文关键词:Lung Neoplasm Radiation Radiosensitivity Cell cycle |
| 基金项目:浙江省医药卫生科研基金资助项目(2000B117);浙江省中医药科研基金资助项目(2002Y002) |
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| 中文摘要: |
| 目的 探讨放射抗拒肺癌细胞系的放射抗拒机理。方法 应用X射线照射肺癌D6细胞系8次,每次吸收剂量650cGy,对存活细胞单克隆化,采用克隆形成实验测定所得的新细胞亚系D6-R细胞及其亲本的放射敏感性,流式细胞术检测它们的细胞周期特征。结果 与亲本D6细胞相比,D6-R细胞显示出放射抗性,其D0、Dq及N值增大,细胞存活曲线肩区增宽,在SF2水平上,D6-R细胞的放射抗性是其亲本细胞D6的1.65倍。D6-R细胞S期比例较其亲本D6细胞明显增高,而G2/M,G1期比例则下降。结论 新的细胞亚系D6-R比其亲本对射线更加抗拒,并且显示出与亲本不同的细胞周期特征。 |
| 英文摘要: |
| Objective To establish a radioresistant cell subline from a human D6 lung cancer cell line and investigate the mechanism of radioresistance. Methods D6 human NSCLC cells were exposed to X-rays generated by a linear accelerator(650 cGy per fraction).After a total exposure dose of 5 200 cGy, a monoclone was obtained.The radiosensitivity and cell cycle distribution of this clone, together with its parent D6 cells, were measured by clonogenic assay and flow cytometry. Results The new clone, namely D6-R subline, had a higher D0(D0=2.08 Gy) and a broader initial shoulder(Dq=1.64 Gy, N=2.20)than those of the parent D6 cell line (D0= 1.84 Gy、Dq=0.34 Gy, N=1.20), being 1.65-fold increase in radioresistance as regards to the SF 2.The D6-R subline also showed higher percentage of cells in S phase(53.4% vs 37.8%), but lower percentages in G1(44.1% vs 57.2%) and G2/M(2.5% vs 5%)phases. Conclusion The new subline D6-R is more radioresistant as compare to its parent D6 cell line, and has a different cell cycle distribution. |
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