中华放射医学与防护杂志

楚建军,苏燎原,许昌韶,等.SHG-44胶质瘤细胞辐射敏感性的测定与实验方法的筛选研究[J].中华放射医学与防护杂志,2003,23(4):255-257.CHU Jianjun,SU Liaoyuan,XU Changshao,et al.A study of radiosensitivity of human glioma cells and accuracy of the experimental methods[J].Chin J Radiol Med Prot,2003,23(4):255-257

SHG-44胶质瘤细胞辐射敏感性的测定与实验方法的筛选研究

A study of radiosensitivity of human glioma cells and accuracy of the experimental methods

投稿时间：2002-10-30

DOI：

中文关键词: 星形胶质细胞瘤细胞存活曲线平板集落形成试验氚标胸腺嘧啶核苷掺入法氮蓝四唑盐比色法

英文关键词:Glioma Cell survival curve Clone forming assay(CFA) 3H-TdR incorporation assay MTT assay

基金项目:

作者	单位
楚建军	214062 无锡, 苏州大学附属第四医院
苏燎原	214062 无锡, 苏州大学核医学院
许昌韶	214062 无锡, 苏州大学附属第一医院
刘芬菊	214062 无锡, 苏州大学核医学院
周菊英	214062 无锡, 苏州大学附属第一医院
章国芬	214062 无锡, 苏州大学附属第四医院
朱南康	214062 无锡, 苏州大学核医学院

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中文摘要:

目的以平板集落形成试验等方法观察人脑胶质瘤SHG-44细胞的辐射敏感特性,并比较细胞存活曲线拟合的回归效果。方法设实验组和对照组,实验组的吸收剂量分别为0.5,1,2,3,4,5,6,7,8,9,10,11,12和13Gy14个剂量点,每点设6个平行样本。以⁶⁰Coγ射线照射,吸收剂量率为2.0Gy/min,实验重复3次,数据取平均值。结果 SHG-44细胞的SF₂为86.96%,D^-2为17.85Gy,其他辐射敏感性指标D₀、D_q、α/β分别为3.20,2.35和16.98Gy。该细胞存活曲线低剂量区存在一个较窄的“肩段”,其倾斜度并非陡峭(1/D₀=0.32)。吸收剂量>10Gy存活率下降较为明显。筛选配比试验分析³H-TdR掺入法的灵敏度为88.5%、特异度为90.0%,但其漏判率和误判率较高均为10%左右。MTT比色法灵敏度为94.2%,但其特异度较差为75.0%,误判率高达25.0%。结论人脑胶质瘤SHG-44细胞具有一定程度的辐射抗性。CFA方法准确、稳定性较好,并且由其拟合的细胞存活曲线效果较佳(Q=0.007,F=4.56,P<0.01)。

英文摘要:

Objective In order to study the radiosensitivity and the regressive effect of cell survival curves in Suzhou human glioma cell-44, clone forming assay (CFA), ³H-TdR incorporation and MTT assay have been applied in the experiment. Methods We established irradiation groups (n=6×14) and control groups (n=12×1).The irradiation groups were divided into 14 subgroups, 6 parallel samples each, irradiated by ⁶⁰Coγ-rays (2.0 Gy/min) with doses of 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 Gy respectively.Each dose group was repeated three times. Results The radiosensitivity values SF₂, D^-2 were 86.96% and 17.85 Gy.The other values D₀ 3.20 Gy, D_q 2.35 Gy and α/β 16.98 Gy were also obtained.The cell survival curve showed a small shoulder in small dose range, and a not steep gradient(1/D₀=0.32).At large doses (>10 Gy) the survival fraction declined rapidly.The sensitivity of ³H-TdR incorporation assays was 90.0%, but specificity was 88.5%.The false-positive and false-negative rates were 100% or so, while the specificity of MTT assay was lower (75.0%), and the false-positive was higher (25.0%). Conclusion Radioresistance appears in SHG-44 cells and the cell survival curve by CFA is the excellent among the three experimental assays (Q=0.007, F=4.56, PP<0.001).

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