中华放射医学与防护杂志

范雁,吴士良,徐岚,等.牛磺酸对受照射白血病细胞株K562中基质金属蛋白酶-2表达的影响[J].中华放射医学与防护杂志,2003,23(4):253-255.FAN Yan,WU Shiliang,XU Lan,et al.Effect of taurine on expressions of MMP-2 in K562 leukaemia cell line exposed to γ-rays[J].Chin J Radiol Med Prot,2003,23(4):253-255

牛磺酸对受照射白血病细胞株K562中基质金属蛋白酶-2表达的影响

Effect of taurine on expressions of MMP-2 in K562 leukaemia cell line exposed to γ-rays

投稿时间：2002-12-16

DOI：

中文关键词: γ射线牛磺酸白血病细胞K562 基质金属蛋白酶-2(MMP-2)

英文关键词:γ-rays Taurine K562 Leukaemia Matrix Metalloproteinase(MMP-2)

基金项目:国防科工委国防预研基金资助项目(Y6920004);江苏省高校自然科学基金资助项目(01KJB310001)

作者	单位	E-mail
范雁	215007 江苏, 苏州大学医学院生化及分子生物学教研室	Shiliangwu@hotmail.com
吴士良	215007 江苏, 苏州大学医学院生化及分子生物学教研室
徐岚	215007 江苏, 苏州大学医学院生化及分子生物学教研室
仇灏	215007 江苏, 苏州大学医学院生化及分子生物学教研室
周迎会	215007 江苏, 苏州大学医学院生化及分子生物学教研室

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中文摘要:

目的探讨γ射线对白血病细胞中基质金属蛋白酶2(MMP-2)表达的影响,并从细胞水平初步探讨牛磺酸对肿瘤细胞受辐照后MMP-2表达的抑制作用。方法加不同剂量牛磺酸的白血病细胞株K562在⁶⁰Coγ射线15Gy照射12h后收集,经凝胶上样缓冲液处理后采用Westernblotting分析各组细胞MMP-2的表达水平。结果 15Gy吸收剂量阳性对照组细胞MMP-2表达量明显增加,阴性对照组1(加药200mgL,不照射)MMP-2表达量较少,加药剂量50mgL组、100mgL组、200mgL组细胞MMP-2表达量依次减少,200mgL组与阴性对照组1细胞MMP-2表达量基本一致。结论 15Gy⁶⁰Coγ射线照射促进白血病细胞K562中MMP-2表达;加不同剂量牛磺酸后对MMP-2表达有抑制作用,且与剂量呈相关性,从而抑制电离辐射引起的肿瘤细胞进一步侵袭和转移,达到生物防治的作用。

英文摘要:

Objective To study the effect of γ-irradiation on expressions of MMP-2 in leukaemia cells and the suppressive effect of taurine(Tau) on irradiated tumour cells in terms of cellular level. Methods The cells in the control group and Tau (50 mg/L, 100 mg/L, 200 mg/L)groups were irradiated with 15 Gy γ-rays. The expressions of MMP-2 were examined through Western-blotting after handled with gel-loading buffer within 12 h. Results The expressions of MMP-2 were enhanced evidently in the positive control group, while they were less in the negative control group. In the Tau(50 mg/L, 100 mg/L, 200 mg/L)groups, the expressions of MMP-2 were diminished in turns, and they were almost identical between the negative control group and the Tau 200 mg/L group. Conclusion Irradiation with γ-rays at a dose of 15 Gy can significantly stimulate the expressions of MMP-2 in K562 cells; Tau can inhibit the expressions of MMP-2 and its effect depends on to its dosage; Tau can inhabit the invasiveness and migration of irradiated tumour cells, so it has the biologic protective and therapeutic effects.

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