中华放射医学与防护杂志

刘晓冬,马淑梅,刘树铮,等.辐射对EL-4、J774A.1细胞CD2、CD48表达的影响[J].中华放射医学与防护杂志,2003,23(4):241-243.LIU Xiaodong,MA Shumei,LIU Shuzheng,et al.Changes of CD2 and CD48 expressions in EL-4 and J774A.1 cell lines after different doses of X-irradiation[J].Chin J Radiol Med Prot,2003,23(4):241-243

辐射对EL-4、J774A.1细胞CD2、CD48表达的影响

Changes of CD2 and CD48 expressions in EL-4 and J774A.1 cell lines after different doses of X-irradiation

投稿时间：2002-09-27

DOI：

中文关键词: 辐射 CD2 CD48 低剂量辐射高剂量辐射

英文关键词:Ionizing irradiation CD2 CD48 LDR HDR

基金项目:国家自然科学基金资助项目(39570188)

作者	单位
刘晓冬	130021 长春, 吉林大学白求恩医学部卫生部放射生物学重点实验室
马淑梅	130021 长春, 吉林大学白求恩医学部卫生部放射生物学重点实验室
刘树铮	130021 长春, 吉林大学白求恩医学部卫生部放射生物学重点实验室
刘扬	130021 长春, 吉林大学白求恩医学部卫生部放射生物学重点实验室

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中文摘要:

目的通过时程及量效研究观察不同剂量X射线照射对EL-4和J774A.1细胞株中表面分子CD2、CD48的影响。方法采用荧光免疫流式细胞术检测蛋白表达的变化。结果 (1)EL-4细胞CD2分子表达结果显示,0.075GyX射线照射后CD2合成在照射后4h即开始升高,至8～16h达峰值(P<0.05～0.01),2Gy照射后则从照射后4h开始下降,至8h达最低点(P<0.01),一直持续较低至48h恢复;8h量效结果显示,在低剂量区0.050,0.075Gy使CD2表达上调,而高剂量范围中1,2Gy使CD2表达下调。(2)J774A.1细胞CD48分子表达结果显示,0.075GyX射线照射后CD48合成在照射后2h即开始升高,至4h达峰值(P<0.05),随后急剧下降,8h恢复至假照射水平,16～48h下降明显低于假照射水平(P<0.05),2Gy照射后则从照射后2h开始下降,至8h达最低点(P<0.01),随后有所恢复,但直至48h仍未能恢复至假照射水平。4h量效结果显示,在低剂量区0.050,0.075,0.100Gy使CD48表达上调,而高剂量范围中1～6Gy均使CD48表达下调(P<0.05～0.01)。结论 X射线照射可引起CD2、CD48不同的辐射效应,两者的相互作用体现出低剂量辐射具有不同于高剂量辐射的兴奋效应。

英文摘要:

Objective To observe the changes in the expressions of CD2 and CD48 in EL-4 and J774A.1 after different doses X-irradiation by time-course and dose-effect studies. Methods Flow cytometry(FCM) was adopted to detect the changes of CD2 and CD8 expressions. Results FCM data showed CD2 expression in EL-4 cells increased beginning from 4 h and reached its peak at 8-16 h after 0.075 Gy irradiation, while 2 Gy decreased its expression from 4 h and reached its minimum at 8 h.Dose-effect results of CD2 expression revealed that 0.050 Gy and 0.075 Gy up-regulated its expression while 1-6 Gy induced reverse changes 8 h after exposure.Simultaneously, CD48 expression in J774A.1 cells showed an earlier increase(4 h) and a later decrease (16-48 h) after 0.075 Gy irradiation, whereas 2 Gy irradiation led to a sustained decrease up to 48 h.Dose-effect studies of CD48 expression showed that 0.050-0.100 Gy up-regulated the expression of CD48 while 1-6 Gy down-regulated its expression significantly. Conclusion The different changes of CD2 in EL-4 cells and CD48 in J774A.1 cells after different doses of X-irradiation indicate that LDR exhibits an opposite effect to that of HDR.

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