| 范保星,张开泰,孙敬芬,等.BEP2D细胞恶性转化不同时期差异表达基因的筛选[J].中华放射医学与防护杂志,2003,23(4):231-233.FAN Baoxing,ZHANG Kaitai,SUN Jingfen,et al.Identification of differentially expressed genes in different malignant transformation phases of BEP2D cell line[J].Chin J Radiol Med Prot,2003,23(4):231-233 |
| BEP2D细胞恶性转化不同时期差异表达基因的筛选 |
| Identification of differentially expressed genes in different malignant transformation phases of BEP2D cell line |
| 投稿时间：2003-01-06 |
| DOI： |
| 中文关键词: α粒子 抑制消减杂交 cDNA芯片 永生化人支气管上皮细胞 |
| 英文关键词:Alpha-particles SSH cDNA microarray BEP2D cell |
| 基金项目:国家重点基础研究发展规划973基金资助项目(G1998051208) |
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| 中文摘要: |
| 目的 筛选并鉴定α粒子辐射诱发永生化人支气管上皮细胞(BEP2D)恶性转化不同时期差异表达的基因。方法 抑制消减杂交(SSH),cDNA芯片。结果 利用SSH构建了BEP2D细胞3个恶性转化不同时期差异表达基因的文库,BEP2D细胞的文库有416个克隆,R15H20细胞的文库有301个克隆,R15H35细胞的文库有586个克隆,经cDNA Microarray验证发现:BEP2D细胞文库来源的芯片与3代细胞探针杂交后的阳性信号率为90.4%、21.6%和19.7%;R15H20细胞文库来源的芯片的阳性信号率为8.6%、93.8%和31.6%:R15H35细胞文库来源的芯片的阳性信号率为23.5%、18.2%和90.7%。结论 联合应用SSH和cDNA Microarray是筛选和鉴定不同样本中差异表达基因的快速和有效方法;经cDNA Microarray鉴定出的3个文库中差异表达的cDNA可能代表了α粒子诱发人支气管上皮细胞恶性转化相关的基因。 |
| 英文摘要: |
| Objective To screen and identify differentially expressed genes in different malignant transformation phased of BEP2D cell line induced by alpha-particles. Methods Suppression subtractive hybridization(SSH) and cDNA microarray were performed. Results Three suppression subtractive cDNA libraries were constructed.BEP2D cDNA library contained 416 clones, R15H20 cDNA library contained 301 clones, and R15H35 cDNA library contained 586 clones.After confirmed by cDNA microarray, the positive rates in BEP2D cDNA microarray were 90.4%, 21.6% and 19.7%;in R15H20 cDNA microarray, 8.6%, 93.8% and 31.6%;and in R15H35 cDNA microarray, 23.5%, 18.2%, 90.7% when hybridized with probes coming from BEP2D, R15H20 and R15H35 cells, respectively. Conclusions The combination of SSH and cDNA microarray is a rapid and effective method for high throughout screening and identification of differentially expressed genes in different samples.The cDNAs confirmed by cDNA microarray may be the relative genes that associate with malignant transformation of bronchial epithelial cells induced by alpha-particles. |
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