| 张军宁,朱寿彭,洪承皎.中性单细胞电泳检测肿瘤细胞放射敏感性[J].中华放射医学与防护杂志,2003,23(3):166-168 |
| 中性单细胞电泳检测肿瘤细胞放射敏感性 |
| A primary investigation on relationship between intrinsic radiosensitivity and radiation-induced initial DNA double-strand breaks(DSBs),DSB repair in three tumor cell lines |
| 投稿时间：2002-01-25 |
| DOI： |
| 中文关键词: 肿瘤细胞内在放射敏感性 中性单细胞凝胶电泳 DNA双链断裂与修复 |
| 英文关键词:Intrinsic radiosensitivity Neutral comet assay DNA double-strand breakange and repair |
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| 中文摘要: |
| 目的 探讨中性单细胞电泳检测肿瘤细胞放射敏感性的可行性。方法 应用克隆形成法和中性单细胞电泳技术,检测辐射诱导的人红白血病细胞株K562、人结肠腺癌细胞株LS-T-117和鼠胶质瘤细胞株C6的初始DNA双链断裂数及双链断裂后的修复,以及两者与细胞放射敏感性之间的关系。结果 3种细胞系的放射敏感性依次为K562>LS-T-117>C6;3种细胞系的DNA迁移长度都随着照射剂量的增加而增大,呈良好的剂量效应关系。在相同剂量下辐射诱导的DNA双链的初始断裂数目,也依次为K562>LS-T-117>C6;3种细胞系经10GyX射线照射,并在PBS中培养不同时间后DNA迁移长度,都有较大幅度的下降,但下降幅度依次为C6>LS-T-117>K562。在相同剂量下辐射诱导的DNA双链断裂后的修复能力,也依次为C6>LS-T-117>K562。结论 辐射诱导的DNA双链断裂及修复,与细胞放射敏感性有很好的相关性,可望用于肿瘤细胞内在放射敏感性的预测。 |
| 英文摘要: |
| Objective To explore the possibility of radiation-induced DNA double-strand breaks (DSBs) detected by neutral comet assay as a predictor of intrinsic radiosensitivity. Method The relationship between intrinsic radiosensitivity of tumor cells and radiation-induced initial DNA DSBs, DSB repair in three tumor cell lines:K562, LS-T-117 and C6 were investigated by the neutral comet assay. Result It was found that there was a close correlation between initial DSBs and the delivered dose:that is the larger the dose, the severer the DSB.For same single dose, the different initial DSBs and intrinsic radiosensitivity measured by means of a clonogenic assay were ranked in the following descending order:K562>LS-T-117>C6, while the capacity of DBS repair:C6>LS-T-117>K562. Conclusion The results suggest both radiation-induced initial DSBs and DSB repair correlate with intrinsic radiosensitivity to a certain extent, which can be used as potential predictors of intrinsic radiosensitivity for tumor cells. |
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