| 马淑梅,刘晓冬,鞠桂芝.电离辐射对小鼠骨髓细胞cyclinB1、cdc2基因转录的影响[J].中华放射医学与防护杂志,2003,23(3):154-156 |
| 电离辐射对小鼠骨髓细胞cyclinB1、cdc2基因转录的影响 |
| Effect of whole body irradiation with X-rays on cyclinB1,cdc2 gene transcription of bone marrow cells in mice |
| 投稿时间：2002-05-19 |
| DOI： |
| 中文关键词: 电离辐射 骨髓细胞 cyclinB1 G2阻滞 cdc2 |
| 英文关键词:Ionizing radiation Bone marrow cells CyclinB1 G2 arrest cdc 2 |
| 基金项目:国家自然科学基金资助项目(39770193) |
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| 中文摘要: |
| 目的 研究X射线全身照射对小鼠骨髓细胞cyclinB1、cdc2基因转录的影响,以探讨电离辐射诱导小鼠骨髓细胞G2期阻滞的分子机理。方法 采用流式细胞仪检测了X射线全身照射后小鼠骨髓细胞周期进程的变化。采用半定量RTPCR方法分别检测cyclinB1、cdc2基因转录水平变化。结果 2GyX射线全身照射后4及12hG2+M期细胞百分率升高(P<0.05);0.5～6Gy照射后12hG2+M细胞百分率呈剂量依赖性增加(P<0.05～P<0.01)。2GyX射线照射后2～48h小鼠骨髓细胞cyclinB1转录水平在各个时间点未见明显变化,0.5～6GyX射线照射后12h,骨髓细胞cyclinB1基因转录在各剂量点均未见明显改变;2GyX射线照射后小鼠骨髓细胞cdc2转录水平从照后4h即开始不同程度下降,12h达最低值(为假照射组的46%),照射后48h开始恢复,0.5～6GyX射线照射后12h,骨髓细胞cdc2基因转录水平在各剂量点均明显降低,分别达到假照射组的40%～70%。结论 电离辐射可诱导小鼠骨髓细胞发生G2期阻滞,cdc2激酶活性下降在G2期阻滞中起重要作用。 |
| 英文摘要: |
| Objective For elucidating the molecular mechanism of G2 arrest induced by ionizing radiation, cyclinB1, cdc2 gene transcriptions of bone marrow cells in mice after whole body irradiation(WBI) were studied. Method Semi-quantitative RT-PCR was used to detect cyclinB1, cdc2 mRNA levels, flow cytometry (FCM) was adopted to analyze the cell cycle progression in Kunming mice following WBI with X-rays. Results The data showed an elevation of the percentage of cells in G2 phase at 4 h and 12 h (P<0.05) and an increase of cells in G2+M phase at 12 h after 0.5-6.0 Gy irradiation in a dose-dependent manner.CyclinB1 showed no changes at mRNA level regardless of 2 Gy irradiation in time-course study or 0.5-6.0 Gy in dose-effect study.The cdc2 mRNA level decreased from 4 h, reached its minimum at 12 h (46% of that of sham-irradiation) and began to recover at 48 h.Dose-effect results of cdc2 showed a decrease of cdc2 mRNA level after 0.5-6.0 Gy. Conclusion cdc2 rather than cyclinB1 seems to play important roles in radiation-induced G2 arrest. |
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