| 李秀娟,李修义,田梅,杨巍.pEgr-ssEndostatin重组质粒瘤内辐射诱导表达及其抗肿瘤作用[J].中华放射医学与防护杂志,2003,23(3):148-150 |
| pEgr-ssEndostatin重组质粒瘤内辐射诱导表达及其抗肿瘤作用 |
| Expression of pEgr-ssEndostatin in tumor induced by radiation and its anti-tumor effect |
| 投稿时间：2002-09-10 |
| DOI： |
| 中文关键词: pEgr-ssEndostatin重组质粒 基因放射治疗 X射线照射 B16黑色素瘤 |
| 英文关键词:pEgr-ssEndostatin recombinant plasmids Gene-radiotherapy X-ray irradiation B16 melanoma |
| 基金项目:国家自然科学基金资助项目(30170290) |
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| 中文摘要: |
| 目的 观察pEgr-ssEndostatin基因放射治疗对移植黑色素瘤B16小鼠的抗肿瘤作用及其在瘤内辐射诱导表达。方法 肿瘤局部注射脂质体包裹的pEgr-ssEndostatin重组质粒后42h,接受20GyX射线照射,观察放疗后不同时间肿瘤大小,检测放疗后第2天瘤内Endostatin转录水平。结果 pEgr-ssEndostatin基因放射治疗荷瘤小鼠能够增强放疗疗效;单纯接种pEgr-ssEndostatin质粒组和接种pEgr-ssEndostatin质粒+20Gy照射组小鼠的肿瘤组织内有Endostatin mRNA表达,且pEgr-ssEndostatin质粒照射组的Endostatin mRNA水平高于单纯pEgr-ssEndostatin质粒组。结论 pEgr-En-dostatin基因-放射治疗抗肿瘤作用明显优于单纯放疗和单纯基因治疗,肿瘤局部转染重组质粒后照射瘤内Endostatin表达增强。 |
| 英文摘要: |
| Objective To observe the antitumor effect of pEgr-ssEndostatin gene-radiotherapy to murine melanoma and the expression of Endostatin gene in tumor induced by radiation. Methods pEgr-ssEndostatin plasmids were injected locally into the tumor in B16 mice, and 42 h later, the tumors of half of the mice were given 20 Gy X-irradiation.The tumor growth at different times and Endostatin expression 2 days after irradiation were detected. Results Endostatin gene-radiotherapy could enhance the curative effect of radiotherapy.Both pEgr-ssEndostatin group and pEgr-ssEndostatin+20 Gy group had Endostatin mRNA expression, but the mRNA level of pEgr-ssEndostatin+20 Gy group was higher than that of pEgr-ssEndostatin group. Conclusion The antitumor effect of pEgr-ssEndostatin gene-radiotherapy is better than that of radiotherapy and gene therapy only.And Endostatin expression in tumor is enhanced after irradiation. |
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