中华放射医学与防护杂志

谷庆阳,王德文,杨志祥,等.急性放射性皮肤溃疡PDGF-B及受体PDGFR-β的表达水平[J].中华放射医学与防护杂志,2003,23(2):86-88.GU Qing yang,WANG Dewen,YANG Zhixiang,et al.Expression of PDGF-B and PDGFR-β during the formation and development of acute radiation-induced skin ulcers and their effects on ulcer healing:a comparative study with normal wound healing[J].Chin J Radiol Med Prot,2003,23(2):86-88

急性放射性皮肤溃疡PDGF-B及受体PDGFR-β的表达水平

Expression of PDGF-B and PDGFR-β during the formation and development of acute radiation-induced skin ulcers and their effects on ulcer healing:a comparative study with normal wound healing

投稿时间：2002-05-11

DOI：

中文关键词: 放射性皮肤溃疡血小板源生长因子-B 血小板源生长因子受体-β 伤口愈合

英文关键词:Radiation-induced skin ulcder PDGF-B PDGFR -β Wound healing

基金项目:全军“九·五”医药卫生科研基金课题(96Q036)

作者	单位
谷庆阳	100850, 北京放射医学研究所
王德文	100850, 北京放射医学研究所
杨志祥	军事医学科学院附属医院
王晓民	100850, 北京放射医学研究所
高亚兵	100850, 北京放射医学研究所
杨红	100850, 北京放射医学研究所
刘杰	100850, 北京放射医学研究所
赵梅兰	100850, 北京放射医学研究所
崔玉芳	100850, 北京放射医学研究所
崔雪梅	100850, 北京放射医学研究所

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中文摘要:

目的研究血小板源生长因子-B(PDGF-B)及其受体血小板源生长因子受体β(PDGFR-β),在急性放射性皮肤溃疡组织中的表达水平及对溃疡形成、发展、愈合的影响。方法采用雌性Wistar大鼠,以⁶⁰Coγ射线局部照射法建立急性放射性皮肤溃疡动物模型,并以手术法建立单纯皮肤伤口动物模型,观察病变55d,采用免疫组化、原位杂交和图像分析等方法检测单纯伤口及皮肤溃疡组织中PDGF-B及PDGFR-β的转录和表达水平。结果照射后14d照射野内开始出现皮肤溃疡,之后逐渐扩大、融合、加深。皮肤受照射区多种细胞,特别是溃疡床表皮细胞、成纤维细胞及血管内皮细胞中PDGF-B及PDGFR-β的转录和表达水平均较正常皮肤组织有所增强,但与单纯伤口组比较,溃疡组织中PDGF-B及PDGFR-β的转录和表达水平明显降低。结论辐射诱导的皮肤溃疡组织中PDGF-B及PDGFR-β的表达水平降低可能与溃疡发生、发展及难愈合的分子机理相关。

英文摘要:

Objective To study the expression of PDGF-B and PDGFR-β during the formation and development of radiation-induced skin ulcers and their effects on ulcer healing. Methods A rat model which was locally irradiated with ⁶⁰Co γ-rays was used, and another rat model of surgical skin wounds was made.Pathological changes were observed for 55 days.Immunohistochemistry, in situ hybridization and image analysis were performed to detect PDGF-B and PDGFR-β expression in the tissue of radiation skin ulcers and surgical wounds. Results Skin ulcers were found on day 14 after irradiation, and they were enlarged and deepened gradually during the observation period.In the irradiated skin, especially in the epidermal cells, fibroblasts and endothelial cells of the ulcer beds, the expressions of PDGF-B and PDGFR-β were higher than those in normal skin.But their expression were obviously decreased in the radiation ulcer beds compared to those in the surgical wound beds. Conclusion After irradiation, the decreased expressions of PDGF-B and PDGFR-β may play an important role in the formation, development and nonhealing mechanism of radiation skin ulcers.

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