中华放射医学与防护杂志

刘青杰,陈德清,陈晓宁,等.多色荧光原位杂交技术的建立及其在早先受照射者剂量重建中的应用[J].中华放射医学与防护杂志,2003,23(2):77-82.LIU Qingjie,CHEN Xiaoning,JIANG Enhai,et al.Establishment of a multicolor FISH method and its application in retrospective dose estimation[J].Chin J Radiol Med Prot,2003,23(2):77-82

多色荧光原位杂交技术的建立及其在早先受照射者剂量重建中的应用

Establishment of a multicolor FISH method and its application in retrospective dose estimation

投稿时间：2002-05-21

DOI：

中文关键词: 多色荧光原位杂交端粒和着丝粒探针易位早先受辐射照射剂量重建

英文关键词:Multicolor FISH Telomere and centromere probes Translocation Previous radiation exposure Dose reconstruction

基金项目:卫生部科学研究基金资助项目(98-2-46);美国能源部基金资助项目(DE-FC03-96ER62294)

作者	单位
刘青杰	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所
陈德清	100088 北京, 中国疾病预防控制中心辐射防护与核安全医学所
陈晓宁	Cedars Sinai Medical Center, UCLA, USA
JulieR.Korenberg	Cedars Sinai Medical Center, UCLA, USA
姜恩海	中国医学科学院放射医学研究所

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中文摘要:

目的建立多色荧光原位杂交方法,并探讨用该方法进行早先辐射受照射者的剂量重建的可行性。方法筛选8对染色体端粒和着丝粒特异性人工细菌染色体(BAC)克隆,建立多色荧光原位杂交方法,用该方法分析⁶⁰Coγ射线离体照射的新生儿脐带血淋巴细胞染色体畸变,并建立相应的标准剂量效应曲线,参照标准剂量效应曲线来估算两例早先受照射者的累积吸收剂量。结果本研究中建立的多色荧光原位杂交方法用生物素和(或)地高辛将端粒和着丝粒BACDNA标记成绿、红、黄(绿+红)3种荧光染色,使得1,2,3,7,8,9,14和15号染色体很容易辨认。用该方法分析受⁶⁰Coγ射线照射的新生儿脐带血淋巴细胞染色体畸变,除断片外,其他染色体畸变的剂量效应曲线均为线性二次剂量反应模式。用所有细胞或稳定性细胞中的完全相互易位率作指标,估算了两例早先受射照者的吸收剂量。结论本研究所建立的多色荧光原位杂交方法可以用来进行早先受照射者的剂量重建。

英文摘要:

Objective To establish an multicolor FISH (M-FISH) method and to investigate its applicability in retrospective dose reconstruction. Methods Bacterial artificial chromosome (BAC) clones specific for telomeres and centromeres of 8 pairs of chromosomes were selected, and an M-FISH method was to be established.Chromosome aberrations in lymphocytes of two new-borns umbilical cord blood having been irradiated with ⁶⁰Co γ-rays in vitro were analyzed with the M-FISH method.Then the dose-response curves of chromosome aberrations were established.The cumulative doses for two individuals having been exposed to ionizing radiation were estimated with the M-FISH method according to the corresponding dose response curve. Results An M-FISH method, using BAC clones specific for telomeres and centromeres of chromosomes 1, 2, 3, 7, 8, 9, 14 and 15, labeled with biotin-14-dATP and/or digoxigenin-11-dUTP, was established.The 8 pairs of chromosomes could be easily distinguished.The dose-response curve between chromosome aberrations rate and the absorbed dose levels for lymphocytes in umbilical cord blood were of linear-quadratic dose-response model, except for fragments without centromere.The cumulative dose estimated for the two individuals were similar with reciprocal translocation rate in all analyzed cells or in stable cells. Conclusion Dose reconstruction of previous radiation exposure victims can be carried out with the M-FISH method established in this study.

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