| 铁轶,董燕,毛建平,等.中国人甲状腺乳头状癌中ret基因重排及类型的分析研究[J].中华放射医学与防护杂志,2003,23(2):73-76.TIE Yi,SONG Guang,Dong Yan,et al.Analysis of ret gene rearrangements and forms in papillary thyroid carcinomas (PTC) of a Chinese population[J].Chin J Radiol Med Prot,2003,23(2):73-76 |
| 中国人甲状腺乳头状癌中ret基因重排及类型的分析研究 |
| Analysis of ret gene rearrangements and forms in papillary thyroid carcinomas (PTC) of a Chinese population |
| 投稿时间：2002-10-23 |
| DOI： |
| 中文关键词: 甲状腺乳头状癌 ret基因 基因重排 RT-PCR |
| 英文关键词:Papillary thyroid carcinomas ret gene Gene arrangement RT-PCR |
| 基金项目:国家自然科学基金资助项目(39970233) |
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| 摘要点击次数: 3709 |
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| 中文摘要: |
| 目的建立ret基因重排的分析方法,提示中国人甲状腺乳头状癌中是否存在原癌基因ret重排及重排类型。方法应用RT-PCR方法对65例甲状腺乳头状癌石蜡切片标本进行ret基因重排检测,并对扩增结果进行测序鉴定。结果在提取RNA成功的38例甲状腺乳头状癌(PTC)标本中,有71%发生ret重排,其中10.5%表达PTC1;5.3%表达PTC3;5.3%表达PTC4,以PTC1发生的频率较高。仅1例表达PTC2与PTC3PTC4混合型重排,中国人PTC可能与ret和H4及ELE1重排激活相关。结论中国人PTC组织中存在4种重排,且以PTC1较为常见。单一标本中有多种重排形式并存,PTC1+3重排形式10例、3例PTC1+4、1例PTC2+3+4、1例PTC3+4、4例PTC1+3+4,单一标本中多种重排形式并存的比例高达50%,但与癌症的恶性程度、转移等无必然联系。 |
| 英文摘要: |
| Objective To establish a method of detecting ret gene arrangements and to investigate the rearrangements and forms of ret oncogene in papillary thyroid carcinomas (PTC) of a Chinese population. Methods RT-PCR was performed to detect the ret arrangements of paraffin-embeded specimens of 65 patients with PTC and the amplified products were cloned into T vector and sequenced using specific primers for ret/PTC 1, 2, 3 and 4. Result RNA was successfully extracted from 38/65 cases, of which 71% were found expressing ret/PTC, including 10.5% PTC1, 5.3% PTC3 and 5.3% PTC4.The frequency of PTC1 was significantly higher than that of PTC2, which was found only in one patient.It was indicated that PTC in the Chinese population was related with ret rearrangements with H4 and ELE1.Previous studies also suggested that PTC1 and PTC3 occurred frequently in PTC patients. Conclusions PTC1, 2, 3 and 4 forms are present in Chinese PTC patients, and PTC1 is more frequently found, consistenting with other reports.Furthermore, multiple rearrangements could be present in a single specimen, with a frequency of 50% for all the detected cases.The present data suggest that the multiple rearrangements in a single specimen may be not linked to the degree of malignancy and metastasis. |
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