| 黄定德,陈杞,韩玲,蔡建明,李百龙,黄越承,高建国,孙苏平.蛋白酪氨酸磷酸酶SHP-1、SHP-2在γ射线诱发的小鼠胸腺淋巴瘤中表达的研究[J].中华放射医学与防护杂志,2003,23(1):23-25 |
| 蛋白酪氨酸磷酸酶SHP-1、SHP-2在γ射线诱发的小鼠胸腺淋巴瘤中表达的研究 |
| Study on expression of SH2 domain-containing protein tyrosine phospatase SHP-1 and SHP-2 in γ-ray irradiation -induced thymus lymphoma in mice |
| 投稿时间：2002-03-04 |
| DOI： |
| 中文关键词: 辐射致癌 信号转导 蛋白酪氨酸磷酸酶 |
| 英文关键词:Radiation carcinogenesis Signal transduction Protein tyrosine phosphatase |
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| 中文摘要: |
| 目的 探讨蛋白酪氨酸磷酸酶SHP-1、SHP-2在γ射线体外诱发的小鼠胸腺淋巴瘤中的表达情况,为从细胞信号转导途径入手研究辐射致癌的分子机理奠定一定的基础。方法 338只BALBc小鼠随机分为照射组及对照组,照射组经60Coγ射线全身照射,病理证实出现胸腺淋巴瘤者为癌变组,未出现任何肿瘤者为未癌变组;对照组不经γ射线照射,与照射组同步喂养。分离胸腺细胞,应用Western blot方法检测其SHP-1及SHP-2蛋白质的表达。结果 癌变组SHP-1蛋白(吸光度A值为15.27)的表达明显,而对照组(吸光度A值为3.80)及未癌变组(吸光度A值为1.01)表达非常弱甚至无表达;SHP-2蛋白的表达总体上呈现癌变组(吸光度A值为2.88)明显高于对照组(吸光度A值为0.33)及未癌变组(吸光度A值为0.99),未癌变组略高于对照组的趋势;应用Western blot分析SHP-2的表达时,癌变组除了SHP-2蛋白表达明显外,同时出现另外1条表达明显的条带,相对分子质量约55×103,该蛋白的表达总体上明显高于对照组及未癌变组。结论 蛋白酪氨酸磷酸酶SHP-1、SHP-2在γ射线诱发的小鼠胸腺淋巴瘤中表达明显增强,说明SHP-1、SHP-2可能与辐射致癌的发生有关,但有待进一步通过实验证实。 |
| 英文摘要: |
| Objective To investigate the expression of SH2 domain containing-protein tyrosine phosphatase SHP-1 and SHP-2 in γ-ray irradiation-induced thymus lymphoma in mice. Methods Altogether 338 BALB/c mice were randomly divided into irradiation groups and controls.Irradiation groups which were irradiated with γ-rays included canceration groups confirmed with histology and uncanceration groups.The controls were fed synchronistically with irradiation groups.The expression of SHP-1 and SHP-2 was detected with Western blot in thymus cells. Results The expression of SHP-1 in canceration groups was much higher than that in uncanceration groups and controls significantly, while the expression of SHP-2 in uncaneration groups was higher than that in uncanceration groups and controls.When we detected the expression of SHP-2 with Western blot, we found another protein with a molecular weight of 55×103, which expression in canceration groups was higer than that in uncanceration groups and controls. Conclusion The expression of SH2 domain-containing protein tyrosine phosphatase SHP-1 and SHP-2 is significantly increased in canceration groups, suggesting that SHP-1 and SHP-2 may be related with γ-ray induced thymus lymphoma in mice.Further research is expected on the relationship between deve-lopment of cancer and SHP-1 and SHP-2. |
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