| 朱寿彭,肖东,吴越.浓缩铀诱发人白血病细胞凋亡及相关基因调控研究[J].中华放射医学与防护杂志,2003,23(1):17-19 |
| 浓缩铀诱发人白血病细胞凋亡及相关基因调控研究 |
| Study on apoptosis in HL-60 cells and its related gene regulation induced by enriched uranium |
| 投稿时间：2002-03-20 |
| DOI： |
| 中文关键词: 浓缩铀235U HL-60细胞 凋亡 基因 |
| 英文关键词:Enriched uranium 235U HL-60 cells Apoptosis Gene |
| 基金项目:国家核工业基金资助项目(Y7197Q6206) |
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| 中文摘要: |
| 目的 研究浓缩铀诱发人白血病HL-60细胞凋亡的损伤特征及对凋亡相关基因bcl-2和bax的调控作用。方法 研究受浓缩铀内照射不同时间诱发HL-60细胞凋亡的DNA凝胶电泳。运用免疫组织化学技术探讨浓缩铀对HL-60细胞bcl-2bax的蛋白表达,以及RNA分子杂交技术探讨浓缩铀对HL-60细胞bcl-2mRNA的转录水平表达。结果 在浓缩铀作用下,HL-60细胞的DNA呈现出在核小体间断裂的阶梯状条带形成的凋亡特征;并且观察到对照HL-60细胞中bcl-2蛋白呈高度表达,为(88±7)%,而受浓缩铀辐照后,可下调至(61±5)%.bax在对照细胞中的表达很低,在浓缩铀作用下,未见明显改变。而且受浓缩铀辐照后,可使HL-60细胞中bcl-2mRNA表达呈明显下调。结论 浓缩铀可诱发HL-60细胞凋亡发生,其诱发凋亡的程度随浓缩铀作用时间的延长而增加。并且发现浓缩铀诱发人白血病HL-60细胞的凋亡作用,与其下调凋亡相关基因bcl-2的表达相关联。 |
| 英文摘要: |
| Objective To study characteristics of injury in apoptosis of HL-60 cells and its related genes bcl-2 and bax expression induced by enriched uranium 235U. Methods DNA gel electrophoresis was used to show the apoptosis of HL-60 cells.Immunohistochemical technique was used to identity the expression of bcl-2 and bax proteins in HL-60 cells, meanwhile RNA dot blot hybridization was used to show the expression of bcl-2 mRNA in HL-60 cells. Results HL-60 cells irradiated with 235U displayed characteristics of DNA ladder formation in apoptotic cells.While the expression of bcl-2 protein was as high as (88±7)% in unirradiated control cells, down-regulation [lowered to (61±5)%] of its expression was noted after exposure to 235U.The expression of bax protein was low in control cells, and after irradiation it did not change obviously.A marked down-regulation of bcl-2 mRNA expression was found in irradiated HL-60 cells. Conclusion Progression of apoptosis in HL-60 cells induced by 235U is dependent on the time elapse of 235U irradiation.The apoptotic action of 235U in HL-60 cells is associated with down-regulation of the apoptosis-related gene bcl-2. |
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