| 马淑梅,刘晓冬,鞠桂芝,刘树铮.电离辐射对小鼠骨髓细胞p53、waf1、gadd45基因转录水平的影响[J].中华放射医学与防护杂志,2003,23(1):11-13 |
| 电离辐射对小鼠骨髓细胞p53、waf1、gadd45基因转录水平的影响 |
| Effect of whole-body X-irradiation on p53,waf1,gadd45 gene transcription of bone marrow cells in mice |
| 投稿时间：2002-03-19 |
| DOI： |
| 中文关键词: 电离辐射 骨髓细胞 p53 waf1 gadd45 |
| 英文关键词:Ionizing radiation Bone marrow cells p53 waf1 gadd45 |
| 基金项目:国家自然科学基金资助项目(39770193) |
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| 中文摘要: |
| 目的 研究X射线全身照射对小鼠骨髓细胞p53、waf1、gadd45基因转录水平的影响,以探讨电离辐射诱导小鼠骨髓细胞G1阻滞的分子机理。方法 采用Northern blot和半定量RT-PCR方法分别检测p53、waf1、gadd45基因转录水平变化。结果 2GyX射线全身照射后2～72hp53基因转录水平均有不同程度的升高,waf1基因在照射后2h开始升高,4h达峰值,一直持续到照后48h开始恢复,gadd45转录水平除在照射后2h一过性升高外,从8h即开始不同程度下降,照射后72h开始恢复。分别以0.5～6.0GyX射线全身照射后12h取小鼠骨髓细胞作量效研究表明,p53的改变在照射后各组虽有不同程度的升高,但未见明显的剂量依赖,waf1基因转录呈明显的剂量依赖性升高,6.0GyX射线照射后达对照组的3.41倍,gadd45未见升高趋势。结论 在电离辐射诱导的小鼠骨髓细胞周期G1阻滞中p53-waf1通路起重要作用,而gadd45可能不参与该过程调控。 |
| 英文摘要: |
| Objective For elucidating the molecular mechanism of G1 arrest induced by ionizing radiation, the transcription of p53, waf1 and gadd45 genes in irradiated bone marrow cells of mice was studied. Methods Northern blot and semi-quantitative RT-PCR were used to detect p53, waf1 and gadd45 at mRNA level. Results The results showed the p53 expression level elevated and waf1 was also up-regulated beginning from 2 h after irradiation and reached its peak at 4 h.As for gadd45, a transient increase was showed at 2 h, then decreased until 72 h.Dose-effect results showed that p53 increased 12 h after whole-body irradiation with 0 .5-6. 0 Gy irradiation, but was not in a significant dose-dependent manner.waf1 showed a dose-dependent increase, 6. 0 Gy might elevate waf1 3.41 times of that in sham-irradiatied mice gadd45 had no significant changes. Conclusion The results suggested that p53 and waf1 other than gadd45 might play important roles in G1 arrest induced by high dose radiation. |
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