| 鞠桂芝,王晓梅,付士波,刘树铮.电离辐射对p16基因转录及蛋白表达的影响[J].中华放射医学与防护杂志,2003,23(1):4-6 |
| 电离辐射对p16基因转录及蛋白表达的影响 |
| Effect of ionizing radiation on p16 gene transcription and protein expression |
| 投稿时间：2002-06-03 |
| DOI： |
| 中文关键词: 电离辐射 p16mRNA p16蛋白表达 |
| 英文关键词:Ionizing radiation p16mRNA p16 protein expression |
| 基金项目:国家自然科学基金资助项目(39770193) |
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| 中文摘要: |
| 目的 研究电离辐射对p16基因转录及蛋白表达的影响。方法 采用Northern blot检测p16mRNA水平的变化;采用单克隆抗体免疫荧光标记及流式细胞术检测p16蛋白表达的变化。结果 研究证实,2.0Gy照射后2～24h,胸腺细胞p16mRNA水平明显增高,8～48hp16蛋白表达显着增高(P<0.05～P<0.01);照射后2～8h脾细胞p16mRNA水平明显增高,24hp16蛋白表达显着增高(P<0.05).研究还证实,0.5～6.0Gy照射后,胸腺细胞p16mRNA水平呈剂量依赖性增高,p16蛋白表达在1.0～4.0Gy组显着增高(P<0.05～P<0.01);脾细胞p16mRNA水平亦增高,但增幅远低于胸腺细胞,p16蛋白表达在1.0～4.0Gy组显着增高(P<0.05～P<0.01).结论 电离辐射可诱导p16基因转录及蛋白表达增高,其增高幅度表现出一定的细胞异质性。 |
| 英文摘要: |
| Objective To investigate the effect of ionizing radiation on p16 gene transcription and protein expression. Methods Northern blot was employed to determine p16mRNA level. Flowcytometry was used to measure p16 protein expression. Results It was demonstrated that p16mRNA level was markedly increased at 2-24 h in thymocytes and at 2-8 h in splenocytes, and p16 protein expression increased significantly at 8-48 h for thymocytes ( P<0.05 to P<0.01) and at 24 h for splenocytes ( P< 0.05) after whole body X-irradiation with 2.0 Gy in mice.In the dose-effect experiments, it was found that p16mRNA level increased with the increase of dose in thymocytes and splenocytes and the p16 protein expression was also increased significantly with the doses from 1.0 to 4.0 Gy for both thymocytes and splenocytes (P<0.05 to P<0.01). Conclusion p16 gene transcription and portein expression could be up-regulated by X-rays and the tendency of increase varied with the radiation dose and cell type. |
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