| 李华,刘雯,李锦燕,左伋.小剂量电离辐射引起拓扑异构酶Ⅱα表达的变化[J].中华放射医学与防护杂志,2003,23(1):1-3 |
| 小剂量电离辐射引起拓扑异构酶Ⅱα表达的变化 |
| Effection of low dose ionizing radiation on expression of DNA topoisomerase Ⅱα |
| 投稿时间：2002-03-21 |
| DOI： |
| 中文关键词: 小剂量电离辐射 拓扑异构酶Ⅱα 基因表达 |
| 英文关键词:Low dose ionizing radiation Topoisomerase Ⅱα Gene expression |
| 基金项目:国家自然科学基金资助项目(30100037) |
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| 中文摘要: |
| 目的 探讨小剂量电离辐射引起DNA拓扑异构酶Ⅱα(TOPOⅡα)的表达变化。方法 用免疫细胞化学染色和Western blot的方法,检测了HeLa细胞在5Gyγ射线照射后的时间效应和小于5Gy的剂量效应,以及小剂量的多次累积效应。结果 5Gyγ射线照射后4hTOPOⅡα开始下降,12h降为最低,16h开始回升;在小于或等于5Gy时,随剂量增加,TOPOⅡα的表达下降逐渐明显;且较小剂量具多次累积效应。结论 小剂量的电离辐射引起的TOPOⅡα的表达变化具时间效应、剂量效应和多次累积效应。 |
| 英文摘要: |
| Objective To explore the changes of topoisomerase (TOPO) Ⅱα expression, the behavior of TOPO Ⅱα in HeLa cells treated with low dose ionizing radiation was studied. Methods The kinetics of TOPO Ⅱα was measured by immunocytochemistry and Western blot. Results A profound and transient decrease of TOPOⅡα after γ irradiation with 5 Gy was detected, the decrease began from 4 h after irradiation, reached the lowest level at 12 h, and began to return at 16 h.Given low dose γ-rays, the level decreased along with the increase of dose.The level of decrease after several times irradiation was higher than that after only one time. Conclusions This observation indicates that the decrease of TOPO Ⅱα after γ-irradiation is time-dependent and dose-dependent, and the effect of low dose γ irradiation can be accumulated. |
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