| 邹跃,杜维霞,张智杰,等.γ射线诱导细胞死亡过程中端粒酶活性的研究[J].中华放射医学与防护杂志,2002,22(6):409-411.ZHOU Yue,DU Weixia,ZHANG Zhijie,et al.Study on telomerase activity during radiation-induced cells death[J].Chin J Radiol Med Prot,2002,22(6):409-411 |
| γ射线诱导细胞死亡过程中端粒酶活性的研究 |
| Study on telomerase activity during radiation-induced cells death |
| 投稿时间：2002-04-18 |
| DOI： |
| 中文关键词: 端粒酶 放射线 细胞死亡 人肿瘤细胞 人正常细胞 |
| 英文关键词:Telomerase Radiation Cell death Human tumor cell line Human normal cell line |
| 基金项目:第二炮兵医药卫生科研计划基金资助项目(9915) |
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| 中文摘要: |
| 目的 观察γ射线照射人肿瘤细胞和正常细胞后端粒酶的变化及其与细胞死亡的关系。方法 检测照射后不同时间细胞端粒酶活性,细胞内端粒酶原位表达以及死亡细胞总数和形态观察。结果 在1～5Gy吸收剂量范围内A549和L02细胞辐射诱导死亡以凋亡为主要途径;在凋亡发生的过程中,端粒酶原位检测显示60%～90%的细胞中存在强烈的端粒酶信号,TRAP检测示端粒酶活性表达增强,提示放射损伤可诱导端粒酶活性增高,高端粒酶活性状态可持续至细胞发生凋亡。结论 γ射线诱导人肿瘤和正常细胞凋亡可能不是通过直接抑制端粒酶活性的机理;辐射诱导端粒酶活性增高可能是细胞修复辐射损伤的机理之一。 |
| 英文摘要: |
| Objective To investigate changes of telomerase activity in human tumor and normal cell lines exposed to radiation and its association with cell death. Methods Telomerase activity was assayed,telomerase in situ hybridization was performed,dead cells were nubmered,and morphology of the dead cells was distinguished at different time points after irradiation. Results Apoptosis was the primary cause of cell death of A549 and L02 cell lines.In the development of apoptosis,positive cells by telomerase in situ hybridization accounted for 60% to 90% of the two lines,and the expression intensity of telomerase activity assayed by TRAP increased,suggesting that radiation injury could induce telomerase activity and that the high level of telomerase activity might be maintained till the developent of aopotosis. Conclusion Telomerase suppression may not be involved in radiation-induced human tumor and normal cell apoptosis and telomerase participates in the process of chromosomal repair. ; |
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