| 霍艳英,张开泰,李邦印,等.TGF-β1在人支气管上皮细胞恶性转化中的作用[J].中华放射医学与防护杂志,2002,22(6):397-400.HUO Yan-ying,ZHANG Kaitai,LI Bangyin,et al.The mechanism of TGF-β1 in radiation-induced human bronchial epithelium tumorigenesis[J].Chin J Radiol Med Prot,2002,22(6):397-400 |
| TGF-β1在人支气管上皮细胞恶性转化中的作用 |
| The mechanism of TGF-β1 in radiation-induced human bronchial epithelium tumorigenesis |
| 投稿时间：2002-04-03 |
| DOI： |
| 中文关键词: TGF-β1 生长 增殖 恶性转化 |
| 英文关键词:TGF-β1 Growth Proliferation Malignant transformation |
| 基金项目:国家重点基础研究发展规划973项目(G1998051207) |
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| 摘要点击次数: 2939 |
| 全文下载次数: 1992 |
| 中文摘要: |
| 目的 研究人支气管上皮细胞恶性转化过程中,TGF-β1表达水平的变化及其对细胞生长、增殖的影响,为进一步研究辐射诱发肿瘤发生的机理奠定基础。方法 在24孔板中按5×104细胞/孔分别接种对数生长期的BEP2D及BERP35T-2细胞,用一定浓度的TGF-β1处理,计数,绘制细胞生长曲线,同时于光镜下观察细胞形态及生长状况。用westernblot方法检测胞浆内及分泌到培养液中的TGF-β1的丰度。结果两种细胞胞浆内的TGF-β1表达水平无明显差异,但BERP35T-2细胞分泌到培养液中的TGF-β1丰度约为BEP2D细胞的5倍。TGF-β1对两种细胞的生长均有抑制作用。在相同的浓度下,TGF-β1对BERP35T-2细胞的生长抑制作用较BEP2D细胞弱。结论 在辐射诱发人支气管上皮细胞恶性转化过程中TGF-β1对其生长抑制作用减弱。 |
| 英文摘要: |
| Objective To study the mechanism of radiation-induced tumorigenesis,the authors analyzed the expression of TGF-β1 in a human bronchial epithelium cell line BEP2D and its α-particle-induced malignantly transformed cell line BERP35T-2 cells,determined the influence of its expression on the cell growth and proliferation,and investigated the effect of exogenous TGF-β1 on these cells. Methods To determining the cell growth rate,5×10 4 cells were plated into 24-well culture plates and treated with 1 ng/ml or 2 ng/ml TGF-β1.The number of cells in three wells was counted with a hemocytometer every day.Proteins were extracted from BEP2D and BERP35T-2 cells or from the supernatant.The abundance of TGF-β1 was examined with Western blot. Results When analyzed the abundance of TGF-β1 in the cytoplasm,TGF-β1 in BERP35T-2 cells was not significantly higher than that in BEP2D cells ( P>0.05 ).As the abundance of TGF-β1 secreted into the culture medium was compared,TGF-β1 from BERP35T-2 cells was significantly higher than that from BEP2D cells( P <0.01).When treated with TGF-β1,inhibitory effect on growth and proliferation of both BEP2D and BERP35T-2 cells was showed. But,the effect of TGF-β1 on BERP35T-2 cells was weaker than that on BEP2D cells. Conclusion In the malignant transformation process of human bronchial epithelial cells induced by radiation,the inhibitory effect of TGF-β1 on cell growth decreased. ; |
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