| 杨光,刘长安,贾廷珍,等.低剂量辐射对脐血免疫细胞功能影响的初步研究[J].中华放射医学与防护杂志,2002,22(6):394-396.YANG Guang,LIU Chang-an,JIA Tingzhen,et al.Effect of low dose radiation on immune function of cord blood cells:A preliminary investigation[J].Chin J Radiol Med Prot,2002,22(6):394-396 |
| 低剂量辐射对脐血免疫细胞功能影响的初步研究 |
| Effect of low dose radiation on immune function of cord blood cells:A preliminary investigation |
| 投稿时间：2001-12-11 |
| DOI： |
| 中文关键词: 低剂量辐射 脐血 自然杀伤细胞 增殖 |
| 英文关键词:Low dose irradiation Cord blood Natural killer cell Lymphocytes |
| 基金项目:国家自然科学基金资助项目(39770238) |
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| 摘要点击次数: 4724 |
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| 中文摘要: |
| 目的 观察低剂量辐射对脐血淋巴细胞增殖、CD25分子表达、IL-2水平和NK细胞活性的影响。方法 ①采用3H-TdR掺入法和3HTdR释放法检测62mGy、124mGy、186mGyγ射线照射新鲜分离的脐血淋巴细胞增殖和NK细胞活性。②采用单克隆抗体直接免疫荧光标记流式细胞术和酶联免疫吸附实验检测62mGyγ射线照射新鲜分离的脐血淋巴细胞在不同时间(12h,24h,48h)CD25分子表达和培养上清液的IL-2水平。结果 在一定剂量范围内的低剂量辐射显着促进脐血淋巴细胞增殖和增强NK细胞活性。62mGyγ射线照射后脐血淋巴细胞CD25表达和IL-2水平显着上调,CD25表达和IL-2的水平随时间推移逐步增高。结论 在一定剂量范围内的低剂量辐射能促进淋巴细胞的增殖、活化和成熟,增强NK细胞活性。低剂量辐射对脐血免疫细胞存在兴奋效应。可能加速脐血移植时造血功能和免疫功能的重建,这可能增加移植物抗白血病效应,降低其复发,显示出临床应用前景。 |
| 英文摘要: |
| Objective To investigate the effects of low dose radiation (LDR) on lymphocyte proliferation,CD25 expression,interleukin-2 (IL-2) level,and natural killer cell(NK cell) activity in cord blood. Methods (1)Isolated mononuclear cells from cord blood were irradiatied with different doses (62 mGy,124 mGy,186 mGy) of gamma-rays.The lymphocyte proliferation and NK cell activity were measured with 3H-TdR incorpation assay and 3H-TdR release assay,respectively.(2)Isolated fresh fresh mononuclear cells from cord blood were irradiated with 62 mGy gamma-rays.At different culture times (12 h,24 h,48 h) after irradiation,CD 25 expression was measured by flow cytometry with direct immunofluoresence and IL-2 levels in the supernatant was measured by ELISA. Results As compared with controls,LDR could promote lymphocyte proliferation and enhance NK cell activity.The CD25 expression and the IL-2 level in cord blood were up-regulated significantly after 62 mGy gamma-ray irradiation and showed increasing amplitude with time. Conclusion LDR may promote proliferation,maturity and activation of lymphocyte and enhance NK cell activity in cord blood,which may contribute to the enhanced graft versus leukemia(GVL) response and the decrease of the recurrence rate of tumor,as well as acceleration of the reconstruction of immune and hematopietic functions after cord blood transplantation,using low dose γ-irradiated mononuclear cells from cord blood is of value in clinical practice. ; |
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