| 史剑慧,许小平,程文英,等.糖皮质激素抑制辐射诱导P388细胞NF-κB活化[J].中华放射医学与防护杂志,2002,22(6):391-393.SHI Jianhui,XU Xiaoping,CHENG Wenying,et al.Inhibitory effects of glucocorticoid on apoptosis and activation of NF-κB in P388 cells induced by radiation[J].Chin J Radiol Med Prot,2002,22(6):391-393 |
| 糖皮质激素抑制辐射诱导P388细胞NF-κB活化 |
| Inhibitory effects of glucocorticoid on apoptosis and activation of NF-κB in P388 cells induced by radiation |
| 投稿时间：2002-01-19 |
| DOI： |
| 中文关键词: 辐射 P388 核因子-κB 凋亡 |
| 英文关键词:Radiation P388 NF-κB Apoptosis |
| 基金项目:国家自然科学基金资助项目(39770330) |
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| 摘要点击次数: 5198 |
| 全文下载次数: 2582 |
| 中文摘要: |
| 目的 研究糖皮质激素对辐射诱导P388白血病细胞凋亡与NF-κB活化的影响。方法 采用TdT介导的dUTP缺口末端标记技术(TUNEL)检测细胞凋亡;采用电泳迁移率变动分析(EMSA)检测P388细胞NFκB活化水平。结果 2、4、6和8Gy辐射能诱导P388细胞凋亡,同时使NFκB活化。地塞米松(DXM)增强由2、4、6和8Gy辐射诱导的P388细胞凋亡,抑制由2,4,6和8Gy辐射诱导的P388细胞NFκB活化,凋亡增加率分别为60%、100%、129%和67%,NF-κB活化抑制率分别为25%、45%、52%和40%.结论 辐射诱导P388白血病细胞凋亡的同时激活NF-κB;糖皮质激素可通过抑制NFκB活化,增强其诱导白血病细胞凋亡的作用。 |
| 英文摘要: |
| Objective To explore effects of glucocorticoid on apoptosis and activation of NF-κB in P388 cells induced by radiation. Methods Apoptosis in P388 cells induced by radiation treatment was detected by TUNEL assay.EMSA was used to detect the activation of NF-κB. Results The apoptosis and activation of NF-κB in P388 cells could be induced by radiation.Dexamethasone (DXM) which could suppress activation of NF-κB of P388 cells increased significantly the apoptosis induced by radiation.Apoptosic rates in DXM-treated P388 cells after 2,4,6 and 8 Gy exposure increased by 60%,100%,129% and 67%,respectively.Activation rates of NF-κB in DXM-treated P388 cells after 2,4,6,and 8 Gy exposure decreased by 25%,45%,52% and 40%,respectively. Conclusion Radiation induces apoptosis and activation of NF-κB in P388 cells simultaneously.Glucocorticoid enhances apoptosis in leukemic cells,which may be by means of suppressing activation of NF-κB. ; |
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