| 秦全红,王德文,彭瑞云,等.照射复合创伤愈合伤口成纤维细胞TGFβ1、TGFβR1蛋白及TGFβ1mRNA的表达与意义[J].中华放射医学与防护杂志,2002,22(5):330-333.QIN Quanhong,WANG Dewen,PENG Ruiyun,et al.Expression of TGFβ1 and TGFβR1 in fibroblasts during healing of combined radiation wound[J].Chin J Radiol Med Prot,2002,22(5):330-333 |
| 照射复合创伤愈合伤口成纤维细胞TGFβ1、TGFβR1蛋白及TGFβ1mRNA的表达与意义 |
| Expression of TGFβ1 and TGFβR1 in fibroblasts during healing of combined radiation wound |
| 投稿时间：2001-10-25 |
| DOI： |
| 中文关键词: 伤口愈合 成纤维细胞(Fb) TGFβ1 TGFβR1 图像分析 |
| 英文关键词:Wound healing Fibroblasts TGFβ1 TGFβ R1 Image analysis |
| 基金项目:全军九五重点招标课题资助项目(96Z008) |
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| 中文摘要: |
| 目的 探讨局部照射对创伤愈合过程中伤口(Fb)TGFβ1及TGFβR1表达的影响及意义。方法 将132只Wistar大鼠分为单纯创伤组(对照组)和照射复合创伤组(照射组),用免疫组织化学、原位杂交和图像分析方法检测两组伤口愈合过程中伤后1～60d各时间点伤口成纤维细胞中TGFβ1、TGFβR1蛋白及TGFβ1 mRNA表达的变化规律。结果 单纯创伤组伤口成纤维细胞TGFβ1、TGFβR1表达有明显的时相性,伤后2～21d可见表达,10d为表达高峰；照射复合创伤组其表达减弱,且表达时相延长,伤后28d仍有表达。结论 照射抑制伤口成纤维细胞内源性TGFβR1及TGFβR1的表达,表明照射使成纤维细胞自分泌TGFβR1功能减弱并对外源性TGFβR1的反应性减弱。 |
| 英文摘要: |
| Objective In order to study the impact of local irradiation on expressions of TGFβ1 and TGFβ R1 in fibroblasts during wound healing. Methods Altogether 132 Wistar rats were divided into two groups:unirradiated wound group (control group, n = 66) and γ-rays irradiated wound group (irradiation group, n =66).We investigated the expression patterns of TGFβ1 and TGFβ R1 in these two groups 1 to 60 days after injury by immunohistochemistry, in situ hybridization and image analysis. Results The expressions of TGFβ1 and TGFβ R1 in the control group had a temporal character,i.e.they were observed from 2 to 21 days after injury and the highest expression was on the 10 th day.In irradiation group,their expressions were weaker than those in the control group and the expression time was prolonged,i.e.,it was still observed on the 28 th day after injury. Conclusion Irradiation with γ-rays can reduce the expressions of TGFβ1 and TGFβ R1 in wound healing fibroblasts,indicating that radiation inhibits the secretion of endogenous TGFβ1 and reduces the reactivity of wound to exogenous TGFβ1. |
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