| 刘光伟,李鹏武,刘淑春,等.低剂量辐射诱导小鼠睾丸生精细胞凋亡的适应性反应[J].中华放射医学与防护杂志,2002,22(5):322-325.LIU Guangwei,LI Pengwu,LIU Shuchun,et al.Adaptive response of spermatogenic cell apoptosis induced by low dose X-ray irradiation in mice[J].Chin J Radiol Med Prot,2002,22(5):322-325 |
| 低剂量辐射诱导小鼠睾丸生精细胞凋亡的适应性反应 |
| Adaptive response of spermatogenic cell apoptosis induced by low dose X-ray irradiation in mice |
| 投稿时间：2001-10-12 |
| DOI： |
| 中文关键词: 低剂量辐射 适应性反应 细胞凋亡 生精细胞 原位末端标记法 |
| 英文关键词:Lowdose irradiation Adaptive response Spoptosis Spermatogenic cell TUNEL |
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| 中文摘要: |
| 目的 研究低剂量电离辐射诱导小鼠睾丸生精细胞凋亡的适应性反应。方法 应用原位末端标记(TUNEL)和常规HE染色法分别结合光镜定量地观察75mGyX射线照射诱导小鼠睾丸生精细胞在生精周期中凋亡的适应性反应。结果 当10、20或3.0Gy(攻击剂量,D2)X射线照射前6h给予75mGy(诱导剂量,D1)预照射时明显减轻D2对睾丸精原细胞和精母细胞的凋亡损伤作用,而对精子细胞影响不明显。当10、15、20、25、30Gy(D2)X射线照射前3、6、12、24h给予75mGy(D1)预照射时,发现当D2剂量较小(10、15、2.0Gy)时,精原细胞和精母细胞的凋亡百分率减少出现较早、较明显,而且持续时间较长；反之,当D2剂量较大(2.5和3.0Gy)时,精原细胞和精母细胞的凋亡百分率减少只在照后6h出现,而且不显着。结论 低剂量电离辐射可以诱导精原细胞和精母细胞凋亡的适应性反应,并与D1和D2间隔时间及D2 |
| 英文摘要: |
| Objective To study the adaptive response of spermatogenic cell apoptosis induced by whole body X ray irradiation with low doses in male Kunming mice. Methods The spermatogenic cell apoptosis induced by 75 mGy X ray irradiation during spermatogenic cell cycle was measured by using the methods of terminal deoxyribonucleotide transferase mediated dUTP bictin nick end labeling (TUNEL) and HE staining. Results When an inductive dose (D1) of 75 mGy was given or not at 6 h before the challenging dose (D2) of 1.0,2.0 or 3.0 Gy,the apoptotic percentages of the spermatogonia and spermatocytes in the D1+D2 groups were declined rapidly as compared with those in the D2 alone groups,and the apoptotic percentages of spermatids showed no significant changes.When the inductive dose (D1) 75 mGy was given at 3,6,12 and 24 h before the challenging doses (D2) of 1.0,1.5,2.0,2.5 and 3.0 Gy,the apoptotic percentages in spermatogonia and spermatocytes reduced early and significantly and continued for a longer duration under smaller D2 doses (1.0,1.5 and 2.0 Gy).However,the apoptotic percentages reduced only at 6 h after whole body irradiation under larger D2 doses (2.5 and 3.0 Gy),and were not significant. Conclusion sThe adaptive response of apoptosis in spermatogonia and spermatocytes could be induced by low doses X ray irradiation.The effect could closely relate to D2 doses and the interval time between D1 and D2. |
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