| 朱一蓓,王月丹,席泓,等.辐射对树突状细胞的影响及其机理的研究[J].中华放射医学与防护杂志,2002,22(5):315-318.ZHU Yibei,WANG Yuedan,XI Hong,et al.The effects of γ-radiation on dendritic cells and its mechanisms[J].Chin J Radiol Med Prot,2002,22(5):315-318 |
| 辐射对树突状细胞的影响及其机理的研究 |
| The effects of γ-radiation on dendritic cells and its mechanisms |
| 投稿时间：2001-12-15 |
| DOI： |
| 中文关键词: γ射线 树突状细胞 生物学效应 |
| 英文关键词:ray Dendritic cells Biological function |
| 基金项目:IAEA协作研究基金项目(CPR9025) |
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| 中文摘要: |
| 目的 研究γ射线对树突状细胞(DCs)生长及其功能的影响。方法 采用γ射线对单核细胞来源的DCs进行照射,经流式细胞仪表型分析、活细胞计数、混合淋巴细胞反应等方法测定γ射线对DCs的生物学效应。结果 γ射线照射抑制DCs的体外增殖,显着地下调DCs表达GM-CS-FR、flt3和gp130分子,但能上调DCs表达CD80、CD86和HLA-DR分子；同时γ射线照射增强DCs激发T细胞的增殖,抑制DCs对抗原摄取的能力。结论 γ射线照射能影响DCs的成熟和功能。 |
| 英文摘要: |
| Objective To study the effects of γ-radiation on dendritic cells(DCs). Methods Monocyte derived DCs were irradiated with γ-ray.Flow cytometric analysis,cell counting,and mixed lymphocyte reaction were used to study the effects of γ-radiation on biological functions of DCs. Results γ-radiation could inhibit the proliferation of DCs in vitro, down regulate the expression of GM CSFR,Flt 3 and gp130,while up regulate CD80,CD86 and HLA DR.Moreover γ-radiation enhanced DCs' potention to activate the proliferation of T cells and reduced DCs' ability for Ag capture. Conclusion γ-radiation may effect the maturation and functions of DCs. |
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