| 杨振国,王德文,宋良文,等.一氧化氮拮抗肺成纤维细胞增殖的量效关系[J].中华放射医学与防护杂志,2002,22(5):311-314.YANG Zhenguo,WANG Dewen,SONG Liangwen,et al.Dose-effect relationship of nitric oxide in inhibiting proliferation of pulmonary fibroblasts[J].Chin J Radiol Med Prot,2002,22(5):311-314 |
| 一氧化氮拮抗肺成纤维细胞增殖的量效关系 |
| Dose-effect relationship of nitric oxide in inhibiting proliferation of pulmonary fibroblasts |
| 投稿时间：2001-11-15 |
| DOI： |
| 中文关键词: 一氧化氮 成纤维细胞 60Coγ射线 增殖 |
| 英文关键词:Nitric oxide Human embryo lung fibroblast 60 Coγray Proliferation |
| 基金项目:国家自然科学基金资助项目(29807213) |
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| 中文摘要: |
| 目的 应用硝普钠(SNP)作为一氧化氮(NO)供体观察NO对60Coγ射线照射的人胚肺成纤维细胞(HELF)增殖活力及其分泌功能的影响,以探讨NO的抗纤维化机理。方法 应用MTT比色法、Griess一氧化氮浓度测定法、免疫组化和原位杂交等方法检测成纤维细胞增殖活性及内皮素(ET)、血管紧张素II(AII)和转化生长因子β(TGFβ)的表达情况。结果 低剂量的60Coγ射线照射对人胚肺成纤维细胞有促增殖作用,以5Gy剂量的促增殖作用最强；30h以内,硝普钠基本以线性方式释放NO,平均增幅为4.25μmolL,初步认为4～5μmolL的NO为抑制成纤维细胞增生的最佳浓度；未照射成纤维细胞不表达或仅微弱表达ET、AII和TGFβ；照后细胞ET和TGFβmRNA及其蛋白表达显着增强,AII合成增加。结论 NO供体硝普钠可明显抑制成纤维细胞的过度增殖和分泌活动,随浓度的加大,抑制作用增强,呈明显的剂量依赖关系。 |
| 英文摘要: |
| Objective To investigate the dose effect relationship of nitric oxide in inhibiting proliferation of pulmonary fibroblasts. Methods MTT colorimetry and immunohistochemistry were used to observe the proliferative activity and expression of ET.TGFβ.AⅡ in fibroblasts. Results Low dose 60 Co γ-ray promoted proliferation of cultured HELF cells especially 5 Gy.NO provider sodium nitroprusside released NO linearly within 30 hours by measuring the concentration of NO in the culture medium.The average increase amplitude was 4.25 μmol/L.The optimum concertration to depress hyperplasia of fibroblast was 4～5 μmol/L.The ET、TGFmRNA transcription and protein expression of ET、TGF.AⅡ were increased after irradiation. Conclusion NO can inhibit excessive proliferation and secretion of fibroblasts in a dose dependent way. |
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