| 席亚明,席亚荣,孙延庆,杜兰宁,刘兆辰,石峰.梁金菇多糖对辐射损伤小鼠造血功能的影响[J].中华放射医学与防护杂志,2002,22(4):291-293 |
| 梁金菇多糖对辐射损伤小鼠造血功能的影响 |
| Experimental studies of the effects of Liang Jin mushroom polysaccharide on hematopoietic function and its mechanism in radiation-injured mice |
| 投稿时间：2001-09-24 |
| DOI： |
| 中文关键词: 梁金菇多糖 辐射损伤 造血细胞 bcl-2蛋白 |
| 英文关键词:Liang Jin mushroom polysaccharide Radiation injury Hematopoietic cells bcl 2 protein |
| 基金项目:甘肃省科委重点基金资助项目(GS981A43149) |
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| 中文摘要: |
| 目的 研究梁金菇多糖(LJPS)对造血功能辐射损伤的治疗作用及机理。方法 用造血祖细胞克隆法,内源性脾结节形成法,骨髓有核细胞计数及脾脏指数等方法观察了LJPS对60Coγ射线照射5.5Gy小鼠造血功能的影响。并用免疫组化法检测辐射骨髓及脾脏细胞的bcl-2蛋白的表达。结果 LJPS辐射后第7天骨髓CFU-GM、CFU-E、CFU-F数量增加,辐射第14天的骨髓有核细胞数、脾脏指数、CFU-S明显增多,辐射第3、7、14天骨髓、脾脏bcl-2蛋白表达上升。结论,LJPS对小鼠造血功能辐射损伤具有治疗作用,其机理与调节骨髓、脾脏bcl-2蛋白表达有关。 |
| 英文摘要: |
| Objective To study the therapeutic effects of Liang Jin mushroom polysaccharide(LJPS)on hematopoietic function and its mechanism in radiation-injured mice. Methods Colony-forming ability of hematopoitic progenitors, colony-forming unit-spleen(CFU-S), number of nucleated cells in bone marrow and spleen index were used to investigate the effects of LJPS on hematopoietic function of mice irradiated with 5.5 Gy 60 Co γ-rays and immunohistochemical technique was used to detect the bcl-2 protein in murine bone marrow and spleenic mononuclear cells. Results LJS could markedly enhance the prolifertion of hematopoietic progenitors and stromal cells in bone marrow of mice irradiated with 5.5 Gy 60 Co γ-rays.On the 14th day after irradiation, compared with the negative control group, CFU-S, spleen index, number of nucleated cells in bone marrow increased markedly in LJPS group, On days 3, 7 and 14, bcl-2 protein level elevated markedly in the LJPS group. Conclusion LJPS has therapeutic effects on irradiated hematopoietic system in mice, and its mechanism is associated with up-regulation of bcl-2 protein in BM and spleen. |
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