| 李津婴,周虹,夏放,翟永平,张雨生,韩风来,章卫平,刘本俶.辐射远后效应对红细胞膜阴离子转运蛋白基因表达的影响[J].中华放射医学与防护杂志,2002,22(4):275-276 |
| 辐射远后效应对红细胞膜阴离子转运蛋白基因表达的影响 |
| The delayed effect of radiation on the gene expression of anion exchanger in erythrocyte membrane |
| 投稿时间：2001-10-20 |
| DOI： |
| 中文关键词: 辐射远后效应 红细胞膜 阴离子转运蛋白 基因表达 |
| 英文关键词:Delayed effect of radiation Erythrocyte membrane Anion exchanger Gene expression |
| 基金项目:总后卫生部专项基金拔款资助项目(98M081) |
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| 全文下载次数: 2022 |
| 中文摘要: |
| 目的 研究辐射远后效应对红细胞膜阴离子转运蛋白(band3)基因表达的影响。方法 分离10年期骨髓型急性放射病(ARS)患者骨髓有核细胞,二步法液体培养红系,取新生红细胞涂片,做band3mRNA原位杂交。抽提外周血有核细胞总RNA,进行RT-PCR,以β-actin作内参照,半定量分析band3mRNA表达丰度。结果 ARS新生红细胞中band3mRNA原位杂交显色不均、淡染,定量分析显示1/2患者band3mRNA表达强度减弱。结论 辐射远后效应可能在转录水平影响红细胞膜阴离子转运蛋白基因表达,使转录产物减少,翻译产物功能下降即阴离子转运能力减弱,红细胞成熟过程延迟。 |
| 英文摘要: |
| Objective To study the delayed effect of radiation on the gene expression of anion exchanger (band 3 protein) in erythrocyte membrane. Methods Two-step liquid culture of erythroid cell was conducted by isolating blasts freshly from patients with bone marrow form of acute radiation sickness (ARS) at the 10 th year after exposure to radiation and the band 3 gene expression in the culture cells was observed using B3 mRNA in situ hybridization.The expression level of band 3 gene in total RNA from peripheral blood mononuclear cells of ARS patients was analyzed by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).β-actin was used as the internal reference. Results Weak and uneven location of band 3 mRNA positive stain in the cultured late erythroblasts was shown by using in situ hybridization.The expression level of band 3 mRNA in half of the ARS patients was decreased. Conclusion The results suggest that the delayed effect of radiation might be exerted on band 3 mRNA transcription, resulting in lowered level of the transcription product and its weakened function, e.g.the decreased anion transport of anion exchanger, and prolonged course of erythropoiesis. |
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