| 孙元明,李进,王芹,唐卫生,王知权.荧光原位杂交法分析中国医用诊断X射线工作者的染色体易位畸变[J].中华放射医学与防护杂志,2002,22(4):269-271 |
| 荧光原位杂交法分析中国医用诊断X射线工作者的染色体易位畸变 |
| Chromosome translocations in Chinese medical X-ray workers analyzed by fluorescence in situ hybridization |
| 投稿时间：2001-12-18 |
| DOI： |
| 中文关键词: X射线工作者 染色体易位 荧光原位杂交 |
| 英文关键词:X-ray workers Chromosome translocation FISH |
| 基金项目:日本体质医学会基金;中国医学科学院中国协和医科大学基金资助项目(952060) |
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| 中文摘要: |
| 目的 用荧光原位杂交技术研究小剂量职业受照者的辐射损伤远期效应。方法 用4#和7#全染色体探针和荧光原位杂交技术,分析25名早期X射线工作者的染色体易位,按照PAINT分类法进行。结果 X射线工作者基因组易位率为(13.14±1.23)1000细胞。完全性易位与不完全性易位为1:1.7.1965年后工作的X射线工作者不完全性易位(Ti)与对照组相比差异有显着性(P<0.05);1965年前工作的X射线工作者不完全性易位(Ti)和完全性易位(Tc)与对照组相比差异有显着性(分别为P<0.01,P<0.05)。结论 医用诊断X射线工作者染色体易位以不完全性易位为主。染色体易位率与染色体DNA含量无关。不完全性易位T（Ab）、T（Ba）和完全性易位Tc均随着放射性工龄的增长而增加。 |
| 英文摘要: |
| Objective To study long-term radiation effect in occupational workers exposed to low dose X-rays using the method of fluorescence in situ hybridization(FISH). Method Chromosome translocations of 25 medical X-ray workers were analyzed by FISH with chromosome 4# and 7# probes according toPAINT (TheProtocol for Aberration Identification and Nomenclature Terminology) system. Results The frequency of genome translocation in X-ray workers was (13.14±1.23)/1 000 cells.The rate of complete and incomplete translocation was 1:1 7.According to the calendar year of entry before/after the year of 1965 as the border, the data showed that the incomplete translocation of the after 1965 group was higher than that of the controls (P <0. 05) and the complete and incomplete translocations of the before 1965 group were obviously higher than those of the controls (P <0. 01 andP <0. 05, respectively). Conclusion The chromosome translocation in early Chinese medical X-ray workers is mainly the incomplete one, the frequency of translocation does not dependent on chromosomal DNA content, and incomplete and comple ones increase along with prolongation of working years in their position. |
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